A molecular toolkit for heterologous protein secretion across Bacteroides species.

Yeh YH, Kelly VW, Rahman Pour R, Sirk SJ
Nat Commun. 2024 Nov 11;15(1):9741. doi: 10.1038/s41467-024-53845-7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
215926	pYHY1	E. coli-Bacteroides shuttle vector.
216108	pYHY2	Constitutive expression of secretory BT0294 SP-NanoLuc via BfP1E6-RBS8 promoter-RBS pair in Bacteroides species.
216109	pYHY3	aTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.