A molecular toolkit for heterologous protein secretion across Bacteroides species.

Yeh YH, Kelly VW, Pour RR, Sirk SJ
bioRxiv. 2023 Dec 15:2023.12.14.571725. doi: 10.1101/2023.12.14.571725. Preprint. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
215926	pYHY1	E. coli-Bacteroides shuttle vector.
216108	pYHY2	Constitutive expression of secretory BT0294 SP-NanoLuc via BfP1E6-RBS8 promoter-RBS pair in Bacteroides species.
216109	pYHY3	aTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.