Combining different CRISPR nucleases for simultaneous knock-in and base editing prevents translocations in multiplex-edited CAR T cells.
Glaser V, Flugel C, Kath J, Du W, Drosdek V, Franke C, Stein M, Pruss A, Schmueck-Henneresse M, Volk HD, Reinke P, Wagner DL
Genome Biol. 2023 Apr 24;24(1):89. doi: 10.1186/s13059-023-02928-7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 215769 | pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated | encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus |