Modular dual-color BiAD sensors for locus-specific readout of epigenome modifications in single cells.
Kohler AR, Hausser J, Harsch A, Bernhardt S, Haussermann L, Brenner LM, Lungu C, Olayioye MA, Bashtrykov P, Jeltsch A
Cell Rep Methods. 2024 Mar 26:100739. doi: 10.1016/j.crmeth.2024.100739.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 215745 | dCas9-10xSunTag(22aa) | Expresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkers |
| 215746 | scFv-IFP2.0N | Expresses single chain Fragment variable (scFv) fused to N-terminal part of split IFP2.0 |
| 215747 | MCP-YPet | Expresses MS2 coat protein (MCP) fused to YPet |
| 215748 | MBD-IFP2.0C | Expresses MBD1 MBD domain fused to C-terminal part of IFP2.0 for 5mC detection in BiAD sensors |
| 215749 | MBD_R44Q-IFP2.0C | Expresses binding-deficient R44Q mutant of MBD1 MBD domain fused to C-terminal part of IFP2.0 as negative control for 5mC detection in BiAD sensors |
| 215750 | 2xHP1bCD-IFP2.0C | Expresses double domain of HP1beta chromo domain fused to C-terminal part of IFP2.0 for H3K9me2/3 detection in BiAD sensors |
| 215751 | 2xHP1bCD_W42A-IFP2.0C | Expresses binding-deficient W42A mutant of 2xHP1beta chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K9me2/3 detection in BiAD sensors |
| 215752 | 2x3APWWP-IFP2.0C | Expresses double domain of DNMT3A PWWP domain fused to C-terminal part of IFP2.0 for H3K36me2/3 detection in BiAD sensors |
| 215753 | 2x3APWWP_K299E_D333A-IFP2.0C | Expresses binding-deficient K299E/D333A mutant of 2xDNMT3A PWWP domain fused to C-terminal part of IFP2.0 as negative control for H3K36me2/3 detection in BiAD sensors |
| 215754 | 2xCBX7CD-IFP2.0C | Expresses double domain of CBX7 chromo domain fused to C-terminal part of IFP2.0 for H3K27me3 detection in BiAD sensors |
| 215755 | 2xCBX7CD_W35A-IFP2.0C | Expresses binding-deficient W35A mutant of 2xCBX7 chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K27me3 detection in BiAD sensors |