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Comprehensive analysis of autophagic functions of WIPI family proteins and their implications for the pathogenesis of beta-propeller associated neurodegeneration.

Shimizu T, Tamura N, Nishimura T, Saito C, Yamamoto H, Mizushima N
Hum Mol Genet. 2023 Aug 7;32(16):2623-2637. doi: 10.1093/hmg/ddad096. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
215506pMRX-IP-muGFP-hATG2A mLIRExpresses GFP tagged human ATG2A with the mutation in LC3 interacting region.
215507pMRX-IP-muGFP-hATG2A ΔWIRExpresses GFP tagged human ATG2A with the mutation in WIPI interacting region.
215508pMRX-IP-muGFP-hATG2A mLIRΔWIRExpresses GFP tagged human ATG2A which has the mutation in LC3 interacting region and lacks WIPI interacting region.
215509pMRX-IP-hWIPI1A-3×FLAGExpresses FLAG tagged human WIPI1A.
215510pMRX-IP-hWIPI2B-3×FLAGExpresses FLAG tagged human WIPI2B.
215511pMRX-IP-hWIPI3-3×FLAGExpresses FLAG tagged human WIPI3.
215512pMRX-IP-hWIPI4-3×FLAGExpresses FLAG tagged human WIPI4.

Antibodies from Article