Sequential CRISPR screening reveals partial NatB inhibition as a strategy to mitigate alpha-synuclein levels in human neurons.
Santhosh Kumar S, Naseri NN, Pather SR, Hallacli E, Ndayisaba A, Buenaventura C, Acosta K, Roof J, Fazelinia H, Spruce LA, Luk K, Khurana V, Rhoades E, Shalem O
Sci Adv. 2024 Feb 9;10(6):eadj4767. doi: 10.1126/sciadv.adj4767. Epub 2024 Feb 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 215486 | FUW-hSNCA-NE | Lentiviral expression of human alpha-synuclein in mammalian cells |
| 215487 | FUW-hSNCA(D2R)-NE | Lentiviral expression of mutated alpha-synuclein (2nd amino acid aspartate mutated to arginine) in mammalian cells |
| 215488 | FUW-hSNCA(D2E)-NE | Lentiviral expression of mutated alpha-synuclein (2nd amino acid aspartate mutated to glutamate) in mammalian cells |
| 215489 | FUW-hSNCA-GFP | Lentiviral expression and easy visualization of alpha-synuclein in mammalian cells |
| 215490 | FUW-hSNCA(D2R)-GFP | Lentiviral expression and easy visualization of mutated alpha-synuclein (2nd amino acid aspartate mutated to arginine) in mammalian cells |
| 215491 | FUW-hSNCA(D2E)-GFP | Lentiviral expression and easy visualization of mutated alpha-synuclein (2nd amino acid aspartate mutated to glutamate) in mammalian cells |