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Natural diversity screening, assay development, and characterization of nylon-6 enzymatic depolymerization.

Bell EL, Rosetto G, Ingraham MA, Ramirez KJ, Lincoln C, Clarke RW, Gado JE, Lilly JL, Kucharzyk KH, Erickson E, Beckham GT
Nat Commun. 2024 Feb 9;15(1):1217. doi: 10.1038/s41467-024-45523-5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
215401AfNitB_pET29bExpression construct for AfNitB gene, codon optimized for expression in E. coli
215402AoC_pET29bExpression construct for AoC gene, codon optimized for expression in E. coli
215403BcC _pET29bExpression construct for BcC gene, codon optimized for expression in E. coli
215404Cut190*_pET21bExpression construct for Cut190* gene, codon optimized for expression in E. coli
215405FsC_pET29bExpression construct for FsC gene, codon optimized for expression in E. coli
215406GatA_pET29bExpression construct for GatA gene, codon optimized for expression in E. coli
215407HotPETase_pET21bExpression construct for HotPETase gene, codon optimized for expression in E. coli
215408LCC-ICCG_pET21bExpression construct for LCC-ICCG gene, codon optimized for expression in E. coli
215409M-NylC_pET28aExpression construct for M-NylC gene, codon optimized for expression in E. coli
215410MGS0156_pET29bExpression construct for MGS0156 gene, codon optimized for expression in E. coli
215411MsmegA _pET29bExpression construct for MsmegA gene, codon optimized for expression in E. coli
215412Nc-NylC_pET28aExpression construct for Nc-NylC gene, codon optimized for expression in E. coli
215413NfPolyA_pET21bExpression construct for NfPolyA gene, codon optimized for expression in E. coli
215414NylB_pET29bExpression construct for NylB gene, codon optimized for expression in E. coli
215415NylB-SCY_pET29bExpression construct for NylB-SCY gene, codon optimized for expression in E. coli
215416NylB'_pET21bExpression construct for NylB' gene, codon optimized for expression in E. coli
215417NylB'-SCY_pET21bExpression construct for NylB'-SCY gene, codon optimized for expression in E. coli
215418NylCA_pET28aExpression construct for NylCA gene, codon optimized for expression in E. coli
215419NylCA-TS_pET28aExpression construct for NylCA-TS gene, codon optimized for expression in E. coli
215420NylCp2_pET28aExpression construct for NylCp2 gene, codon optimized for expression in E. coli
215421NylCp2-TS_pET28aExpression construct for NylCp2-TS gene, codon optimized for expression in E. coli
215422NylCK_pET28aExpression construct for NylCK gene, codon optimized for expression in E. coli
215423NylCK-TS_pET28aExpression construct for NylCK-TS gene, codon optimized for expression in E. coli
215424OoH_pET29bExpression construct for OoH gene, codon optimized for expression in E. coli
215425Ph-NylC_pET28aExpression construct for Ph-NylC gene, codon optimized for expression in E. coli
215426ReU_pET29bExpression construct for ReU gene, codon optimized for expression in E. coli
215427RoL10X_pET29bExpression construct for RoL10X gene, codon optimized for expression in E. coli
215429ThcCut1_pET29bExpression construct for ThcCut1 gene, codon optimized for expression in E. coli
215430ThcCut2 _pET29bExpression construct for ThcCut2 gene, codon optimized for expression in E. coli
215431Tt-NylC_pET28aExpression construct for Tt-NylC gene, codon optimized for expression in E. coli
215432TtA_pET29bExpression construct for TtA gene, codon optimized for expression in E. coli
215433UMG-SP-1_pET29bExpression construct for UMG-SP-1 gene, codon optimized for expression in E. coli
215434UMG-SP-2_pET29bExpression construct for UMG-SP-2 gene, codon optimized for expression in E. coli
215435UMG-SP-3_pET29bExpression construct for UMG-SP-3 gene, codon optimized for expression in E. coli

Antibodies from Article