Detection of fluorescent protein mechanical switching in cellulo.

Shoyer TC, Collins KL, Ham TR, Blanchard AT, Malavade JN, Johns BA, West JL, Hoffman BD
Cell Rep Methods. 2024 Jul 15;4(7):100815. doi: 10.1016/j.crmeth.2024.100815. Epub 2024 Jul 9. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
215371	Actin Binding Domain Tensionless Control	Expresses Actin Binding Domain Tensionless Control (ABDTL), composed of Ftractin-Linker-TSMod. This is the unloaded control for Actin Binding Domain Tension Sensor (ABDTS).
215368	Actin Binding Domain Tension Sensor	Expresses Actin Binding Domain Tension Sensor (ABDTS), composed of Ftractin-Linker-TSMod-Linker-Ftractin. TSMod is the tension sensor module containing mTFP1 and mVenus.