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A lentiviral toolkit to monitor airway epithelial cell differentiation using bioluminescence.

Orr JC, Laali A, Durrenberger PF, Lazarus KA, El Mdawar MB, Janes SM, Hynds RE
Am J Physiol Lung Cell Mol Physiol. 2024 Oct 1;327(4):L587-L599. doi: 10.1152/ajplung.00047.2024. Epub 2024 Aug 13. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
215326pCCL-dNTP63Promoter-FLuc-CMV-RLuc-dsRed2Lentiviral mammalian vector with firefly luciferase reporter for dNTP63 expression; renilla luciferase and dsRed2 reporter under CMV promoter.
215327pCCL-MUC5ACPromoter-FLuc-CMV-RLuc-dsRed2Lentiviral mammalian vector with firefly luciferase reporter for MUC5AC expression; renilla luciferase and dsRed2 reporter under CMV promoter.
215328pCCL-FOXJ1Promoter-FLuc-CMV-RLuc-dsRed2Lentiviral mammalian vector with firefly luciferase reporter for FOXJ1 expression; renilla luciferase and dsRed2 reporter under CMV promoter.

Antibodies from Article