Synthetic Biology Toolbox for Nitrogen-Fixing Soil Microbes.

Venkataraman M, Ynigez-Gutierrez A, Infante V, MacIntyre A, Fernandes-Junior PI, Ane JM, Pfleger B
ACS Synth Biol. 2023 Dec 15;12(12):3623-3634. doi: 10.1021/acssynbio.3c00414. Epub 2023 Nov 21. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
215283	pWN164	Expresses sfGFP under the Anderson J23101 promoter with an RK2 backbone.
215284	pWN165	Expresses sfGFP under the Anderson J23104 promoter with an RK2 backbone.
215291	pWN172	Expresses sfGFP under the Anderson J23113 promoter with an RSF1010 backbone.
215295	pWN212	Expresses sfGFP under the Anderson J23107 promoter with a BBR1 backbone.
215302	pMVV094	Expresses sfGFP under the Anderson J23119 promoter with a pRO1600 backbone.
215303	pMVV052	Expresses sfGFP under rhamnose induction (RhaS + RhaR + pRhaB-sfGFP) with a BBR1 backbone.
215304	pMVV098	Expresses sfGFP under induction with the DAPG Marionette Cassette (PhIFAM + PPhIF-sfGFP) with a BBR1 backbone.
215305	pMVV099	Expresses sfGFP under induction with the Cuminic Acid Marionette Cassette (CymRAM + PCymRC-sfGFP) with a BBR1 backbone.
215306	pMVV100	Expresses sfGFP under induction with the 3OC6-HSL Marionette Cassette (LuxR + PLuxB-sfGFP) with a BBR1 backbone.
215307	pMVV101	Expresses sfGFP under induction with the Vanillic Acid Marionette Cassette (VanRAM + PVanCC-sfGFP) with a BBR1 backbone.
215308	pMVV102	Expresses sfGFP under induction with the IPTG Marionette Cassette (LacIAM + PTac-sfGFP) with a BBR1 backbone.
215309	pMVV103	Expresses sfGFP under induction with the aTc Marionette Cassette (TetR + PTet-sfGFP) with a BBR1 backbone.
215310	pMVV104	Expresses sfGFP under induction with the arabinose Marionette Cassette (AraCAM + araE + PBAD-sfGFP) with a BBR1 backbone.
215311	pMVV105	Expresses sfGFP under induction with the choline Marionette Cassette (BetIAM + PBetI-sfGFP) with a BBR1 backbone.
215312	pMVV106	Expresses sfGFP under induction with the naringenin Marionette Cassette (TtgRAM + PTtG-sfGFP) with a BBR1 backbone.
215337	pMVV107	Expresses sfGFP under induction with the DHBA Marionette Cassette (PcaUAM + P3B5B-sfGFP) with a BBR1 backbone.
215338	pMVV108	Expresses sfGFP under induction with the Salicylic Acid Marionette Cassette (NahRAM + PSalTTC-sfGFP) with a BBR1 backbone.
215339	pMVV109	Expresses sfGFP under induction with the 3OC14-HSL Marionette Cassette (CinRAM + PCin-sfGFP) with a BBR1 backbone.
215340	pMVV110	Expresses sfGFP under induction with the Acrylic Acid Marionette Cassette (acuRAM + PAcu-sfGFP) with a BBR1 backbone.
215341	pMVV111	Expresses sfGFP under induction with the Erythromycin Marionette Cassette (MphRAM + EryR + PMph-sfGFP) with a BBR1 backbone.
215342	pMVV205	Recombineering plasmid with constitutively expressed cas9 and the araBAD promoter expressing αβγ on a BBR1 backbone
215343	pMVV207	gRNA expression for S. stutzeri nifL deletion on an RSF1010 backbone