Synthetic Biology Toolbox for Nitrogen-Fixing Soil Microbes.
Venkataraman M, Ynigez-Gutierrez A, Infante V, MacIntyre A, Fernandes-Junior PI, Ane JM, Pfleger B
ACS Synth Biol. 2023 Dec 15;12(12):3623-3634. doi: 10.1021/acssynbio.3c00414. Epub 2023 Nov 21.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 215283 | pWN164 | Expresses sfGFP under the Anderson J23101 promoter with an RK2 backbone. |
| 215291 | pWN172 | Expresses sfGFP under the Anderson J23113 promoter with an RSF1010 backbone. |
| 215295 | pWN212 | Expresses sfGFP under the Anderson J23107 promoter with a BBR1 backbone. |
| 215302 | pMVV094 | Expresses sfGFP under the Anderson J23119 promoter with a pRO1600 backbone. |
| 215305 | pMVV099 | Expresses sfGFP under induction with the Cuminic Acid Marionette Cassette (CymRAM + PCymRC-sfGFP) with a BBR1 backbone. |
| 215307 | pMVV101 | Expresses sfGFP under induction with the Vanillic Acid Marionette Cassette (VanRAM + PVanCC-sfGFP) with a BBR1 backbone. |
| 215308 | pMVV102 | Expresses sfGFP under induction with the IPTG Marionette Cassette (LacIAM + PTac-sfGFP) with a BBR1 backbone. |
| 215309 | pMVV103 | Expresses sfGFP under induction with the aTc Marionette Cassette (TetR + PTet-sfGFP) with a BBR1 backbone. |
| 215310 | pMVV104 | Expresses sfGFP under induction with the arabinose Marionette Cassette (AraCAM + araE + PBAD-sfGFP) with a BBR1 backbone. |
| 215311 | pMVV105 | Expresses sfGFP under induction with the choline Marionette Cassette (BetIAM + PBetI-sfGFP) with a BBR1 backbone. |
| 215312 | pMVV106 | Expresses sfGFP under induction with the naringenin Marionette Cassette (TtgRAM + PTtG-sfGFP) with a BBR1 backbone. |
| 215338 | pMVV108 | Expresses sfGFP under induction with the Salicylic Acid Marionette Cassette (NahRAM + PSalTTC-sfGFP) with a BBR1 backbone. |
| 215339 | pMVV109 | Expresses sfGFP under induction with the 3OC14-HSL Marionette Cassette (CinRAM + PCin-sfGFP) with a BBR1 backbone. |
| 215341 | pMVV111 | Expresses sfGFP under induction with the Erythromycin Marionette Cassette (MphRAM + EryR + PMph-sfGFP) with a BBR1 backbone. |
| 215342 | pMVV205 | Recombineering plasmid with constitutively expressed cas9 and the araBAD promoter expressing αβγ on a BBR1 backbone |
| 215343 | pMVV207 | gRNA expression for S. stutzeri nifL deletion on an RSF1010 backbone |