A quantitative autonomous bioluminescence reporter system with a wide dynamic range for Plant Synthetic Biology.
Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D
Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. Epub 2023 Oct 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 215242 | GB1531 | TU for constitutive expression of Streptomyces phage phiC31 integrase. Catalyzes site-specific recombination between phiC31 attB and attP sites. Contains SV40 NLS. N. benthamiana codon optimized. |
| 215243 | GB3668 | TU for the expression of mAID (N-tag): AcrIIA4 w/o NLS protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and TNOS terminator |
| 215245 | GB3472 | CDS of H3H from N. nambi codon optimized for N.benthamiana. This gene is the second step of the bioluminiscente pathway |
| 215246 | GB3473 | CDS of Luz gene from N. nambi codon optimized for N.benthamiana. This gene generate the luminiscence of the bioluminiscente pathway |
| 215247 | GB3774 | CDS of CPH from N. nambi codon optimized for N.benthamiana. This gene is the final step and recycling of the bioluminiscente pathway |
| 215251 | GB4193 | TU for the expression of the CIB1 gene fused to the VPR activation domain under the P35S promoter and TNOS terminator in alpha1 |
| 215252 | GB4401 | Module for the expression of the N. nambi HISPS gene under the regulation of PNOS and TNOS and the constitutive expression of CPH, LUZ, H3H, EGFP and p19. |
| 215254 | GB4403 | Module for the expression of the N. nambi HISPS gene under the regulation of PNtA1 and TNOS and the constitutive expression of CPH, LUZ, H3H, EGFP and p19. |
| 215255 | GB4404 | Module for the expression of the N. nambi HISPS gene under the regulation of PNtA11 and TNOS and the constitutive expression of CPH, LUZ, H3H, EGFP and p19 |
| 215256 | GB4405 | Module for the expression of nptII, the N. nambi CPH, LUZ and H3H gene flankeds by insulators on both sides |
| 215257 | GB4468 | Module for the constitutive expression of the N. nambi LUZ, H3H genes, EGFP and p19 fused to a SF in omega2. |
| 215258 | GB4485 | Module for the constitutive expression of the N. nambi CPH, LUZ, H3H genes, EGFP and p19 fused to a SF in alpha2. |
| 215259 | GB4540 | Module for the expression of the N. nambi HISPS gene under the regulation of Solanum lycpersicum DFR promoter and TNOS and the constitutve expression of CPH, LUZ, H3H, EGFP and p19 |
| 215260 | GB4541 | Module for the expression of the N. nambi HISPS gene under the regulation of the Red light-regulated Etr4:mDFR promoter and TNOS in and the constitutve expression of CPH, LUZ, H3H, EGFP and p19. |
| 215261 | GB4542 | Module for the expression of the N. nambi HISPS gene under the regulation of the copper inducible CBS4x:mDFR promoter and TNOS and the constitutve expression of CPH, LUZ, H3H, EGFP and p19 |
| 215264 | GB4636 | Module for the expression of HISPS under the ed light regulated Etr8:CMVmin promoter and H3H under the copper inducible CBS:mDFR promoter and the constitutive expression of LUZ, CPH,EGFP and p19 |
| 215265 | GB4650 | TU for the copper regulated expression of the PhiC31 recombinase. |
| 215266 | GB4652 | TU for the copper regulated expression of the flippase (FLP) recombinase. |
| 215268 | GB4655 | Module for the copper and PhiC31 regulated expression of HispS and the constitutive expression of CPH, LUZ, H3H and eGFP. |
| 215270 | GB4665 | Module for the expression of nptII and the N. nambi LUZ and CPH genes flanked by insulators on the sides of the construction. |
| 215271 | GB4666 | pUPD2 for the Arabidopsis thaliana ABA-responsive MAPKKK18 promoter. |
| 215272 | GB4668 | Module for the expression of the N. nambi HISPS gene under the ABA- responsive MAPKKK18 promoter and the constitutive expression of CPH, LUZ, H3H, EGFP and p19. |
| 215273 | GB4807 | Module for the expressino of the N. nambi HISPS gene under the PNOS promoter and the constitutive expression of EGFP and p19. |
| 215274 | GB4808 | Module for the expressino of the N. nambi HISPS gene under the P35S promoter and the constitutive expression of EGFP and p19. |
| 215276 | GB4832 | Module for the constitutive expression of HISPS, H3H, EGFP and p23. |
| 215369 | GB1203 | TU for the expression of the silencing suppressor P19 driven by the 35s promoter |
| 215370 | GB3474 | CDS of CPH from N. nambi codon optimized for N.benthamiana. This gene is the final step and recycling of the bioluminiscente pathway |