A quantitative autonomous bioluminescence reporter system with a wide dynamic range for Plant Synthetic Biology.
Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D
Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. Epub 2023 Oct 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 215242 | GB1531 | TU for constitutive expression of Streptomyces phage phiC31 integrase. Catalyzes site-specific recombination between phiC31 attB and attP sites. Contains SV40 NLS. N. benthamiana codon optimized. |
| 215243 | GB3668 | TU for the expression of mAID (N-tag): AcrIIA4 w/o NLS protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and TNOS terminator |
| 215245 | GB3472 | CDS of H3H from N. nambi codon optimized for N.benthamiana. This gene is the second step of the bioluminiscente pathway |
| 215246 | GB3473 | CDS of Luz gene from N. nambi codon optimized for N.benthamiana. This gene generate the luminiscence of the bioluminiscente pathway |
| 215251 | GB4193 | TU for the expression of the CIB1 gene fused to the VPR activation domain under the P35S promoter and TNOS terminator in alpha1 |
| 215252 | GB4401 | Module for the expression of the N. nambi HISPS gene under the regulation of PNOS and TNOS and the constitutive expression of CPH, LUZ, H3H, EGFP and p19. |
| 215271 | GB4666 | pUPD2 for the Arabidopsis thaliana ABA-responsive MAPKKK18 promoter. |
| 215369 | GB1203 | TU for the expression of the silencing suppressor P19 driven by the 35s promoter |
| 215370 | GB3474 | CDS of CPH from N. nambi codon optimized for N.benthamiana. This gene is the final step and recycling of the bioluminiscente pathway |