DNA-guided transcription factor cooperativity shapes face and limb mesenchyme.
Kim S, Morgunova E, Naqvi S, Goovaerts S, Bader M, Koska M, Popov A, Luong C, Pogson A, Swigut T, Claes P, Taipale J, Wysocka J
Cell. 2024 Feb 1;187(3):692-711.e26. doi: 10.1016/j.cell.2023.12.032. Epub 2024 Jan 22.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 215022 | pAAV_FKBP-V5-TWIST1 | AAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for human TWIST1 |
| 215023 | pAAV_FKBP-V5-ALX1 | AAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for human ALX1 |
| 215024 | pAAV_FKBP-V5-PRRX1 | AAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for human PRRX1 |
| 215025 | pAAV_MSX1-FKBP-mNeonGreen-V5 | AAV vector for knocking in a C-terminal tag (FKBP12F36V, mNeonGreen, and V5) for human MSX1 |
| 215026 | pGL3-noSV40-humanEC1.45_min1-2_4XEboxMutant | Firefly luciferase enhancer reporter plasmid with minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with E-boxes mutated |
| 215027 | pCAG_TWIST1 | Mammalian expression of human TWIST1 |
| 215028 | pCAG_ALX4-Flag-HA | Mammalian expression of human ALX4 |
| 215029 | pcDNA3.1_ALX4-Flag | Mammalian expression of human ALX4 |
| 215030 | pcDNA3.1_V5-TWIST1 | Mammalian expression of human TWIST1 |
| 215031 | pcDNA3.1_V5-NEUROD1 | Mammalian expression of human NEUROD1 |
| 215032 | pcDNA3.1_V5-TWIST1_NEUROD1loop | Mammalian expression of human TWIST1 (with NEUROD1 loop) |
| 215033 | pcDNA3.1_V5-TWIST1_NEUROG2loop | Mammalian expression of human TWIST1 (with NEUROG2 loop) |
| 215034 | pcDNA3.1_V5-TWIST1_HAND2loop | Mammalian expression of human TWIST1 (with HAND2 loop) |
| 215035 | pcDNA3.1_V5-TWIST1_TAL1loop | Mammalian expression of human TWIST1 (with TAL1 loop) |
| 215036 | pcDNA3.1_V5-TWIST1_NEUROD1ins | Mammalian expression of human TWIST1 (with insertion from NEUROD1) |
| 215037 | pcDNA3.1_V5-TWIST1_NEUROG2ins | Mammalian expression of human TWIST1 (with insertion from NEUROG2) |
| 215038 | pcDNA3.1_V5-TWIST1_HAND2ins | Mammalian expression of human TWIST1 (with insertion from HAND2) |
| 215039 | pcDNA3.1_V5-TWIST1_TAL1ins | Mammalian expression of human TWIST1 (with insertion from TAL1) |
| 215040 | pcDNA3.1_V5-TWIST1_P139S | Mammalian expression of human TWIST1 (with P139S mutation) |
| 215041 | pcDNA3.1_V5-TWIST1_L138Y | Mammalian expression of human TWIST1 (with L138Y mutation) |
| 215042 | pcDNA3.1_V5-TWIST1_L138F | Mammalian expression of human TWIST1 (with L138F mutation) |
| 215043 | pcDNA3.1_V5-TWIST1_NEUROD1L | Mammalian expression of human TWIST1 (with fragment of loop from NEUROD1) |
| 215044 | pcDNA3.1_V5-TWIST1_NEUROD1R | Mammalian expression of human TWIST1 (with fragment of loop from NEUROD1) |
| 215045 | pcDNA3.1_V5-TWIST1_NEUROD1M | Mammalian expression of human TWIST1 (with fragment of loop from NEUROD1) |