Wade Harper Lab: Harper Lab plasmids
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214984 | pcDNA3.1-ccdB-3xFLAG | Gateway compatible empty vector (transient mammalian expression) with a C-terminal 3xFLAG tag |
| 214985 | pcDNA3.1-ccdB-V5 | Gateway compatible empty vector (transient mammalian expression) with a C-terminal V5 tag |
| 214986 | pcDNA3.1-ccdB-3xHA | Gateway compatible empty vector (transient mammalian expression) with a C-terminal 3xHA tag |
| 214988 | pcDNA3.1-ccdB-TwinStrep | Gateway compatible empty vector (transient mammalian expression) with a C-terminal TwinStrep tag |
| 214992 | pHAGE-APEX2-nDEST | Gateway compatible empty vector (lentiviral/mammalian expression) with an N-terminal APEX2 tag |
| 214993 | pHAGE-cDEST-APEX2 | Gateway compatible empty vector (lentiviral/mammalian expression) with a C-terminal APEX2 tag |
| 215001 | pDONR223 MitoFlux (pSu9-HaloTag7-mGFP-STOP) | Shuttling vector for a mitophagy reporter |
| 215002 | pDONR223 ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP) | Shuttling vector for an ERphagy reporter |
| 215003 | pDONR223 LysoFlux (HaloTag7-mGFP-Galectin3-STOP) | Shuttling vector for a Lysophagy reporter |
| 215004 | pDONR223 LC3Flux (HaloTag7-mGFP-LC3-STOP) | Shuttling vector for an autophagy (LC3) reporter |
| 215008 | pLenti UBC LysoFlux (HaloTag7-mGFP-Galectin3) | Express a Lysophagy reporter in mammalian cells (untested) |
| 215009 | pHAGE pGK LysoFlux (HaloTag7-mGFP-Galectin3) | Express a Lysophagy reporter in mammalian cells (untested) |
| 215010 | pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP) | Express a Mitophagy reporter in mammalian cells (untested) |
| 215011 | pHAGE pGK MitoFlux (pSu9-HaloTag7-mGFP-STOP) | Express a Mitophagy reporter in mammalian cells (untested) |
| 215012 | pLenti UBC ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP) | Express a ERphagy reporter in mammalian cells (untested) |
| 215013 | pHAGE pGK ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP) | Express a ERphagy reporter in mammalian cells (untested) |
| 215014 | pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP) | Express an autophagy (LC3) reporter in mammalian cells (untested) |
| 215015 | pHAGE pGK LC3Flux (HaloTag7-mGFP-LC3-STOP) | Express an autophagy (LC3) reporter in mammalian cells (untested) |