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Proteome census upon nutrient stress reveals Golgiphagy membrane receptors.

Hickey KL, Swarup S, Smith IR, Paoli JC, Miguel Whelan E, Paulo JA, Harper JW
Nature. 2023 Nov;623(7985):167-174. doi: 10.1038/s41586-023-06657-6. Epub 2023 Sep 27. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
214969pKH115_GALNT2-keimaGolgiphagy flux reporter GALNT2-keima
214970pKH116_keima-YIPF3Golgiphagy flux reporter keima-YIPF3
214971pKH118_keima-YIPF4Golgiphagy flux reporter keima-YIPF4
214972pKH144_mCherry-YIPF4 WTmCherry tagged YIPF4
214973pKH145_mCherry-YIPF4 LIR mutmCherry tagged LIR mutant YIPF4
214974pKH142_EGFP-YIPF3 WTGFP tagged YIPF3
214975pKH143_EGFP-YIPF3 LIR mutGFP tagged LIR mutant YIPF3
214976pKH151_PB_flag-LC3B_wt_IRES-BFPFlag tagged LC3B
214977pKH152_PB_flag-LC3B_LDSmut_IRES-BFPFlag tagged LDS mutant LC3B
214978pKH159_FLAG-APEX2-YIPF4 WTFlag-APEX2 tagged YIPF4
214979pKH160_FLAG-APEX2-YIPF4 LIR mutFlag-APEX2 tagged LIR mutant YIPF4
214980pKH161_FLAG-APEX2-YIPF3 WTFlag-APEX2 tagged YIPF3
214981pKH162_FLAG-APEX2-YIPF3 LIR mutFlag-APEX2 tagged LIR mutant YIPF3
214982pKH163_pSmart-mNEON-YIPF4endogenous mneon tag for YIPF4

Antibodies from Article