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Simple recombinant monoclonal antibody production from Escherichia coli.

Baker K, Eastwood TA, Garcia E, Lennon C, Mulvihill DP.
Open Biol. 2025 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
214950pRSFDUET-1_VNp-anti-Lysozyme fAb-BiFCBacterial expression of anti-chicken lysozyme Fab light and heavy chain VNp fusions. Each chain is fused to separate halves of a BiFC protein -Venus fluorescence signifies Hc/Lc heterodimer formation.
214951pRSFDUET-1_VNp-anti-Lysozyme fAbBacterial expression of anti-chicken lysozyme (folded) Fab light and heavy chain VNp fusions
214952pRSFDUET-1_VNp-anti-6xHis mAbBacterial expression of anti-6xHis mAb light and heavy chain VNp fusions.
214954pRSFDUET-1_VNp-anti-Muc1 mAbBacterial expression of anti-Muc1 (human) mAb light and heavy chain VNp fusions.
214955pRSFDUET-1_VNp-anti-GFP mAbBacterial expression of anti-GFP mAb light and heavy chain VNp fusions.

Antibodies from Article