Simple recombinant monoclonal antibody production from Escherichia coli.
Baker K, Eastwood TA, Garcia E, Lennon C, Mulvihill DP
Open Biol. 2025 Feb;15(2):240229. doi: 10.1098/rsob.240229. Epub 2025 Feb 19.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214950 | pRSFDUET-1_VNp-anti-Lysozyme fAb-BiFC | Bacterial expression of anti-chicken lysozyme Fab light and heavy chain VNp fusions. Each chain is fused to separate halves of a BiFC protein -Venus fluorescence signifies Hc/Lc heterodimer formation. |
| 214951 | pRSFDUET-1_VNp-anti-Lysozyme fAb | Bacterial expression of anti-chicken lysozyme (folded) Fab light and heavy chain VNp fusions |
| 214952 | pRSFDUET-1_VNp-anti-6xHis mAb | Bacterial expression of anti-6xHis mAb light and heavy chain VNp fusions. |
| 214954 | pRSFDUET-1_VNp-anti-Muc1 mAb | Bacterial expression of anti-Muc1 (human) mAb light and heavy chain VNp fusions. |
| 214955 | pRSFDUET-1_VNp-anti-GFP mAb | Bacterial expression of anti-GFP mAb light and heavy chain VNp fusions. |