Pil1 Co-tethering Assay to Detect Protein-Protein Interactions in the Fission Yeast Schizosaccharomyces pombe.
Pan ZQ, Yang YS, Du LL
Methods Mol Biol. 2025;2862:93-102. doi: 10.1007/978-1-0716-4168-2_7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 215492 | pDB5225 | A pUra4 backbone integrating plasmid expressing Pil1-mCherry under the inducible promoter 41nmt1 |
| 215493 | pDB5226 | A pAde6 backbone integrating plasmid expressing Pil1-mCherry under the inducible promoter 41nmt1 |
| 215494 | pDB5227 | A pLys3 backbone integrating plasmid expressing Pil1-mCherry under the inducible promoter 41nmt1 |
| 215495 | pDB5228 | A pHis5 backbone integrating plasmid expressing Pil1-mCherry under the inducible promoter 41nmt1 |
| 215496 | pDB5230 | A pAde6 backbone integrating plasmid expressing Pil1-mCherry-SpAtg8(1-115) under the inducible promoter 41nmt1 |
| 215497 | pDB4875 | A pUra4 backbone integrating plasmid expressing mECitrine under the inducible promoter 41nmt1 |
| 215498 | pDB4878 | A pAde6 backbone integrating plasmid expressing mECitrine under the inducible promoter 41nmt1 |
| 215499 | pDB4881 | A pLys3 backbone integrating plasmid expressing mECitrine under the inducible promoter 41nmt1 |
| 215500 | pDB4884 | A pHis5 backbone integrating plasmid expressing mECitrine under the inducible promoter 41nmt1 |
| 215501 | pDB5253 | A pUra4 backbone integrating plasmid expressing mECitrine-CtNbr1 under the inducible promoter 41nmt1 |
| 215502 | pDB4887 | A pUra4 backbone integrating plasmid expressing mTurquoise2 under the inducible promoter 41nmt1 |
| 215503 | pDB4890 | A pAde6 backbone integrating plasmid expressing mTurquoise2 under the inducible promoter 41nmt1 |
| 215504 | pDB4893 | A pLys3 backbone integrating plasmid expressing mTurquoise2 under the inducible promoter 41nmt1 |
| 215505 | pDB4896 | A pHis5 backbone integrating plasmid expressing mTurquoise2 under the inducible promoter 41nmt1 |