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Li-Lin Du Lab: Pil1 co-tethering assay to detect protein-protein interactions in the fission yeast Schizosaccharomyces pombe

Unpublished

Plasmids from Article

ID Plasmid Purpose
215492pDB5225A pUra4 backbone integrating plasmid expressing Pil1-mCherry under the inducible promoter 41nmt1
215493pDB5226A pAde6 backbone integrating plasmid expressing Pil1-mCherry under the inducible promoter 41nmt1
215494pDB5227A pLys3 backbone integrating plasmid expressing Pil1-mCherry under the inducible promoter 41nmt1
215495pDB5228A pHis5 backbone integrating plasmid expressing Pil1-mCherry under the inducible promoter 41nmt1
215496pDB5230A pAde6 backbone integrating plasmid expressing Pil1-mCherry-SpAtg8(1-115) under the inducible promoter 41nmt1
215497pDB4875A pUra4 backbone integrating plasmid expressing mECitrine under the inducible promoter 41nmt1
215498pDB4878A pAde6 backbone integrating plasmid expressing mECitrine under the inducible promoter 41nmt1
215499pDB4881A pLys3 backbone integrating plasmid expressing mECitrine under the inducible promoter 41nmt1
215500pDB4884A pHis5 backbone integrating plasmid expressing mECitrine under the inducible promoter 41nmt1
215501pDB5253A pUra4 backbone integrating plasmid expressing mECitrine-CtNbr1 under the inducible promoter 41nmt1
215502pDB4887A pUra4 backbone integrating plasmid expressing mTurquoise2 under the inducible promoter 41nmt1
215503pDB4890A pAde6 backbone integrating plasmid expressing mTurquoise2 under the inducible promoter 41nmt1
215504pDB4893A pLys3 backbone integrating plasmid expressing mTurquoise2 under the inducible promoter 41nmt1
215505pDB4896A pHis5 backbone integrating plasmid expressing mTurquoise2 under the inducible promoter 41nmt1

Antibodies from Article