CRISPR screen for protein inclusion formation uncovers a role for SRRD in the regulation of intermediate filament dynamics and aggresome assembly.
Sweeney KM, Chantarawong S, Barbieri EM, Cajka G, Liu M, Spruce L, Fazelinia H, Portz B, Copley K, Lapidot T, Duhamel L, Greenwald P, Saida N, Shalgi R, Shorter J, Shalem O
PLoS Genet. 2024 Feb 5;20(2):e1011138. doi: 10.1371/journal.pgen.1011138. eCollection 2024 Feb.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214916 | TRE-TDP-43ΔNLS-mClover3 | inducible expression of TDP-43 harboring mutant NLS and C-terminal mClover3. Expression yields cytosolic diffuse TDP-43 |
| 214917 | TRE-mClover3-TDP-43ΔNLS | inducible expression of TDP-43 harboring mutant NLS and N-terminal mClover3. Expression yields cytosolic TDP-43 that forms peinuclear puncta |
| 214918 | EF1a-SRRD-HA (in pLEX_307) | constitutive expression of SRRD tagged with HA |
| 214919 | EF1a-SRRD-noTag (in pLEX_307) | constitutive expression of SRRD with no tag - used as control for SRRD-HA expression |
| 214920 | TRE-SRRD-mRuby3 | inducible expression of SRRD tagged on C-terminus with mRuby3 |
| 214921 | EF1a-SRRD-V5-APEX2 (in pLEX_307) | constitutive expression of SRRD fused to V5 and APEX2 - used for APEX2 proximity biotinylation |
| 214922 | TRE-mClover3 | expression vector control - contitutive expression of mClover3 alone |
| 214923 | TRE-mRuby3 | expression vector control - contitutive expression of mRuby33 alone |