A versatile CRISPR-Cas13d platform for multiplexed transcriptomic regulation and metabolic engineering in primary human T cells.
Tieu V, Sotillo E, Bjelajac JR, Chen C, Malipatlolla M, Guerrero JA, Xu P, Quinn PJ, Fisher C, Klysz D, Mackall CL, Qi LS
Cell. 2024 Feb 29;187(5):1278-1295.e20. doi: 10.1016/j.cell.2024.01.035. Epub 2024 Feb 21.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214875 | pSLQ4419 pHR (hU6-BsmBI-BsmBI-EFS-PuroR-WPRE) | Empty lentiviral backbone for guide array cloning |
| 214876 | pSLQ7501 pHR (hU6-crNT-EFS-PuroR-WPRE) | Lentiviral vector encoding RfxCas13d nontargeting control guide |
| 214877 | pSLQ7529 pHR (hU6-crLPT-EFS-PuroR-WPRE) | Lentiviral vector encoding RfxCas13d targeting LPT guide array |
| 214878 | pSLQ7534 pHR (hU6-crSURF1-EFS-PuroR-WPRE) | Lentiviral vector encoding RfxCas13d targeting SURF1 guide array |
| 214879 | pSLQ7535 pHR (hU6-crSURF2-EFS-PuroR-WPRE) | Lentiviral vector encoding RfxCas13d targeting SURF2 guide array |
| 214880 | pSLQ7556 pHR (hU6-crCD46-EFS-PuroR-WPRE) | Lentiviral vector encoding RfxCas13d targeting CD46 guide |
| 214881 | pSLQ7558 pHR (hU6-crB2M-EFS-PuroR-WPRE) | Lentiviral vector encoding RfxCas13d targeting B2M guide |
| 214882 | pSLQ7578 pHR (hU6-crPROX-EFS-PuroR-WPRE) | Lentiviral vector encoding RfxCas13d targeting PROX guide array |
| 214883 | pSLQ7820 pHR (hU6-crPURI-EFS-PuroR-WPRE) | Lentiviral vector encoding RfxCas13d targeting PURI guide array |
| 214884 | pSLQ7854 pHR (hU6-crGLY-EFS-PuroR-WPRE) | Lentiviral vector encoding RfxCas13d targeting GLY guide array |
| 214885 | pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE) | Lentiviral vector encoding TMP-regulatable RfxCas13d-DD fusion |