Improving prime editing with an endogenous small RNA-binding protein.
Yan J, Oyler-Castrillo P, Ravisankar P, Ward CC, Levesque S, Jing Y, Simpson D, Zhao A, Li H, Yan W, Goudy L, Schmidt R, Solley SC, Gilbert LA, Chan MM, Bauer DE, Marson A, Parsons LR, Adamson B
Nature. 2024 Apr 3. doi: 10.1038/s41586-024-07259-6.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214812 | pCMV-PE7 | Mammalian expression of SpCas9 PE7 prime editor |
| 214813 | pT7-PE7 for IVT | Template for in vitro transcription of PE7 |
| 222996 | pCMV-PE7-P2A-GFP | Mammalian expression of SpCas9 PE7 prime editor with P2A-EGFP marker |
| 222997 | pCMV-PE7-P2A-hMLH1dn | Mammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized) |
| 222998 | pSFFV-IGKleader-hIgG1_FC-Myc-PDGFRb-IRES2-EGFP (MCS reporter) | MCS reporter constitutively expressing EGFP and conditionally expressing a synthetic cell surface protein upon prime editing. For lentivirus packaging. |
| 222999 | pSFFV-EGFP-IRES2-mCherry (FACS reporter) | FACS reporter constitutively expressing mCherry and conditionally expressing EGFP upon prime editing. For lentivirus packaging. |
| 223000 | PEmax_AAVS1_knockin_HDR_donor | HDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm |
| 223001 | PE7_AAVS1_knockin_HDR_donor | HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm |
| 223022 | T7_CC_PE7_IVT_Template | Template for in vitro transcription of PE7. For HSPC-related experiments. |
| 224122 | pCMV-PE7-P2A-BSD | Mammalian expression of SpCas9 PE7 prime editor with P2A-BSD marker |
| 224123 | pCMV-PE7-mutant (Q20A_Y23A_Y24F_F35A) | Mammalian expression of SpCas9 PE7 mutant prime editor (Q20A_Y23A_Y24F_F35A) |