Purification of micrococcal nuclease for use in ribosomal profiling of high-salinity extremophiles.
Gregorova P, Isada M, DiRuggiero J, Sarin LP
J Biol Chem. 2024 Nov 27;301(1):108020. doi: 10.1016/j.jbc.2024.108020.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214808 | pET-28a(+)-T7-OmpA-MNaseA-6xHis | Expression of 6xhis-tagged micrococcal nuclease A (MNaseA) |
| 214809 | pET-28a(+)-T7-OmpA-MNaseB-6xHis | Expression of 6xhis-tagged micrococcal nuclease B (MNaseB) |