Exonuclease-enhanced prime editors.
Truong DJ, Geilenkeuser J, Wendel SV, Wilming JCH, Armbrust N, Binder EMH, Santl TH, Siebenhaar A, Gruber C, Phlairaharn T, Zivanic M, Westmeyer GG
Nat Methods. 2024 Feb 1. doi: 10.1038/s41592-023-02162-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214734 | pCAG_iPE-N | encodes iPE-N prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the N terminus of nCas9-H840A) driven by a CAG promoter |
| 214735 | pCAG_iPE-C | encodes iPE-C prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the C terminus of nCas9-N863A) driven by a CAG promoter |
| 214736 | pCAG_PCP-T5Exo | encodes T5 exonuclease with a PP7 coat protein fused to the N terminus |
| 214737 | AAVS1-eTLR | encodes the enhanced traffic light reporter (eTLR) flanked by homology arms for integration into the AAVS1 safe harbor locus |