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Mutant calreticulin interacts with MPL in the secretion pathway for activation on the cell surface.

Masubuchi N, Araki M, Yang Y, Hayashi E, Imai M, Edahiro Y, Hironaka Y, Mizukami Y, Kihara Y, Takei H, Nudejima M, Koike M, Ohsaka A, Komatsu N
Leukemia. 2020 Feb;34(2):499-509. doi: 10.1038/s41375-019-0564-z. Epub 2019 Aug 28. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
214699pMSCV-IRES-GFP/CALR wtMammalian expression of human CALR wt
214700pMSCV-IRES-GFP/CALR del52Mammalian expression of human CALR del52
214701pMSCV-IRES-GFP/CALR ins5Mammalian expression of human CALR ins5
214706pMSCV-IRES-GFP/CALR ins5 YD/FL-FLAGMammalian expression of human CALR ins5 YD/FL-FLAG

Antibodies from Article