Large-scale evaluation of the ability of RNA-binding proteins to activate exon inclusion.
Schmok JC, Jain M, Street LA, Tankka AT, Schafer D, Her HL, Elmsaouri S, Gosztyla ML, Boyle EA, Jagannatha P, Luo EC, Kwon EJ, Jovanovic M, Yeo GW
Nat Biotechnol. 2024 Jan 2. doi: 10.1038/s41587-023-02014-0.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214672 | lucMAPT-30D | MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs downstream of the 5′ splice site |
| 214673 | lucMAPT-30U | MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs upstream of the 3′ splice site |
| 214674 | lucMAPT-GenRep | MAPT Exon 10 reporter, with Exon 10 and 100bp flanking intronic regions replaced with a BamHI/EcoRI cloning site for testing different exons |
| 214675 | FLAG NC | Negative control plasmid for splicing reporters consisting of MCP fused FLAG epitope tags |