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Kevin Janes Lab: Selectable lentivectors delivering dual gRNAs and destabilized Cas9

Unpublished

Plasmids from Article

ID Plasmid Purpose
214678pDual_dsCas9_VenusLentiviral expression vector for an inducible Cas9-P2A-Venus with two empty sgRNA cloning sites
214679pDual_dsCas9_BFPLentiviral expression vector for an inducible Cas9-P2A-BFP with two empty sgRNA cloning sites
214680pDual_dsCas9_PuroLentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two empty sgRNA cloning sites
214681pDual_dsCas9_BlastLentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two empty sgRNA cloning sites
214682pDual_dsCas9_Venus_hs_NC_chr1Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
214683pDual_dsCas9_Venus_hs_NC_chr2Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 2 (negative control)
214684pDual_dsCas9_Venus_hs_NQO1Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human NQO1
214685pDual_dsCas9_Venus_hs_TP73Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human TP73
214686pDual_dsCas9_BFP_hs_NC_chr1Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
214687pDual_dsCas9_BFP_hs_TP73Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human TP73
214688pDual_dsCas9_Puro_hs_NC_chr1Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
214689pDual_dsCas9_Puro_hs_TP73Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human TP73
214690pDual_dsCas9_Blast_hs_NC_chr1Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
214691pDual_dsCas9_Blast_hs_TP73Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human TP73
214692pDual_dsCas9_Venus_hs_NRF2Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human NRF2

Antibodies from Article