Protocol for identifying properties of ERBB receptor antagonists using the barcoded ERBBprofiler assay.
Popovic L, Rossner MJ, Wehr MC
STAR Protoc. 2024 Apr 16;5(2):102987. doi: 10.1016/j.xpro.2024.102987.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214610 | pcDNA3_EGFR-NTEV-tcs-GV-2xHA | EGFR receptor for split TEV assay |
| 214611 | pcDNA3_ERBB3-NTEV-tcs-GV-2xHA | ERBB3 receptor for split TEV assay |
| 214612 | pcDNA3_ERBB2-V5 | ERBB2 receptor for ERBB2/ERBB3 heterodimer split TEV recuitment assay |
| 214613 | pcDNA3_ERBB4-NTEV-tcs-GV-2xHA | ERBB4 receptor for split TEV assay |
| 214614 | pcDNA3.1_Zeo_3xSH2(GRB2)-CTEV-2xHA | adapter for RTK split TEV recruitment assays |
| 214615 | pTag4C_SP-HTR2A-V2R-NTEV-tcs-GV-2xHA | HTR2A receptor for split TEV assay |
| 214616 | pGL4_10xUAS-CMVmin-BC0372-luc2 | barcoded assay, UAS sensor for split TEV assays |
| 214617 | pGL4_10xUAS-CMVmin-BC0373-luc2 | barcoded assay, UAS sensor for split TEV assays |
| 214618 | pGL4_10xUAS-CMVmin-BC0374-luc2 | barcoded assay, UAS sensor for split TEV assays |
| 214619 | pGL4_10xUAS-CMVmin-BC0376-luc2 | barcoded assay, UAS sensor for split TEV assays |
| 214620 | pGL4_10xUAS-CMVmin-BC0382-luc2 | barcoded assay, UAS sensor for split TEV assays |
| 214621 | pGL4_10xUAS-CMVmin-BC0383-luc2 | barcoded assay, UAS sensor for split TEV assays |
| 214622 | pGL4_10xUAS-CMVmin-BC0385-luc2 | barcoded assay, UAS sensor for split TEV assays |
| 214623 | pGL4_10xUAS-CMVmin-BC0386-luc2 | barcoded assay, UAS sensor for split TEV assays |
| 214624 | pGL4_10xUAS-CMVmin-BC0388-luc2 | barcoded assay, UAS sensor for split TEV assays |
| 214625 | pGL4_10xUAS-CMVmin-BC0389-luc2 | barcoded assay, UAS sensor for split TEV assays |
| 214626 | pGL4_10xUAS-CMVmin-BC0391-luc2 | barcoded assay, UAS sensor for split TEV assays |
| 214627 | pGL4_10xUAS-CMVmin-BC0395-luc2 | barcoded assay, UAS sensor for split TEV assays |
| 214628 | pGL4_10xUAS-CMVmin-BC0401-luc2 | barcoded assay, UAS sensor for split TEV assays |
| 214629 | pGL4_10xUAS-CMVmin-BC0402-luc2 | barcoded assay, UAS sensor for split TEV assays |
| 214630 | pGL4_10xUAS-CMVmin-BC0403-luc2 | barcoded assay, UAS sensor for split TEV assays |
| 214631 | pGL4_10xUAS-CMVmin-BC0404-luc2 | barcoded assay, UAS sensor for split TEV assays |
| 214632 | pGL4_10xUAS-CMVmin-BC0424-luc2 | barcoded assay, UAS sensor for split TEV assays |
| 214633 | pGL4_10xUAS-CMVmin-BC0425-luc2 | barcoded assay, UAS sensor for split TEV assays |
| 214634 | pGL4_10xUAS-CMVmin-BC0427-luc2 | barcoded assay, UAS sensor for split TEV assays |
| 214635 | pGL4_CRE-CMV-BC0482-luc2 | barcoded assay, cAMP/Ca2+ sensor |
| 214636 | pGL4_CRE-CMV-BC0483-luc2 | barcoded assay, cAMP/Ca2+ sensor |
| 214637 | pGL4_CRE-CMV-BC0484-luc2 | barcoded assay, cAMP/Ca2+ sensor |
| 214638 | pGL4_CRE-CMV-BC0487-luc2 | barcoded assay, cAMP/Ca2+ sensor |
| 214639 | pGL4_CRE-CMV-BC0488-luc2 | barcoded assay, cAMP/Ca2+ sensor |
| 214640 | pGL4_CRE-CMV-BC0489-luc2 | barcoded assay, cAMP/Ca2+ sensor |
| 214641 | pGL4_CRE-CMV-BC0490-luc2 | barcoded assay, cAMP/Ca2+ sensor |
| 214642 | pGL4_CRE-CMV-BC0495-luc2 | barcoded assay, cAMP/Ca2+ sensor |
| 214643 | pGL4_CRE-CMV-BC0497-luc2 | barcoded assay, cAMP/Ca2+ sensor |
| 214644 | pGL4_CRE-CMV-BC0502-luc2 | barcoded assay, cAMP/Ca2+ sensor |
| 214645 | pGL4_EGR1p-BC0129-luc2 | barcoded assay, MAPK sensor |
| 214646 | pGL4_EGR1p-BC0130-luc2 | barcoded assay, MAPK sensor |
| 214647 | pGL4_EGR1p-BC0131-luc2 | barcoded assay, MAPK sensor |
| 214648 | pGL4_EGR1p-BC0143-luc2 | barcoded assay, MAPK sensor |
| 214649 | pGL4_EGR1p-BC1485-luc2 | barcoded assay, MAPK sensor |
| 214650 | pGL4_EGR1p-BC1486-luc2 | barcoded assay, MAPK sensor |
| 214651 | pGL4_EGR1p-BC1487-luc2 | barcoded assay, MAPK sensor |
| 214652 | pGL4_EGR1p-BC1488-luc2 | barcoded assay, MAPK sensor |
| 214653 | pGL4_EGR1p-BC1489-luc2 | barcoded assay, MAPK sensor |
| 214654 | pGL4_EGR1p-BC1490-luc2 | barcoded assay, MAPK sensor |
| 214655 | pGL4_NFAT-RE-CMV-BC0525-luc2 | barcoded assay, Ca2+ sensor |
| 214656 | pGL4_NFAT-RE-CMV-BC0526-luc2 | barcoded assay, Ca2+ sensor |
| 214657 | pGL4_NFAT-RE-CMV-BC0527-luc2 | barcoded assay, Ca2+ sensor |
| 214658 | pGL4_NFAT-RE-CMV-BC0528-luc2 | barcoded assay, Ca2+ sensor |
| 214659 | pGL4_NFAT-RE-CMV-BC0529-luc2 | barcoded assay, Ca2+ sensor |
| 214660 | pGL4_NFAT-RE-CMV-BC0530-luc2 | barcoded assay, Ca2+ sensor |
| 214661 | pGL4_NFAT-RE-CMV-BC0533-luc2 | barcoded assay, Ca2+ sensor |
| 214662 | pGL4_NFAT-RE-CMV-BC0537-luc2 | barcoded assay, Ca2+ sensor |
| 214663 | pGL4_NFAT-RE-CMV-BC0538-luc2 | barcoded assay, Ca2+ sensor |
| 214664 | pGL4_NFAT-RE-CMV-BC0546-luc2 | barcoded assay, Ca2+ sensor |
| 214665 | pGL4_NFAT-RE-CMV-BC0547-luc2 | barcoded assay, Ca2+ sensor |
| 214666 | pAAV_MLP-BC0250-luc2 | barcoded assay, internal control |
| 214667 | pAAV_MLP-BC0252-luc2 | barcoded assay, internal control |
| 214668 | pAAV_MLP-BC0253-luc2 | barcoded assay, internal control |
| 214669 | pAAV_MLP-BC1403-luc2 | barcoded assay, internal control |
| 214670 | pAAV_MLP-BC1405-luc2 | barcoded assay, internal control |