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Per-pixel unmixing of spectrally overlapping fluorophores using intra-exposure excitation modulation

Valenta H, Bierbuesse F, Vitale R, Ruckebusch C, Vandenberg W, Dedecker P
Talanta. 2024 Mar (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
214605pcDNA3_pLyn_EGFPExpresses fluorescent protein EGFP targeted to plasma membrane
214606pH2B-6AA-ffDronpaExpresses reversibly switchable fluorescent protein ffDronpa targeted to the nucleus
214607pvimentin-ffDronpaFExpresses reversibly switchable fluorescent protein ffDronpaF targeted to vimentin
214608pffDronpa2F-peroxisomeExpresses reversibly switchable fluorescent protein ffDronpa2F targeted to peroxisome

Antibodies from Article