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Cooperative polarization of MCAM/CD146 and ERM family proteins in melanoma.

Miller SG, Hoh M, Ebmeier CC, Tay JW, Ahn NG
Mol Biol Cell. 2023 Dec 20:mbcE23060255. doi: 10.1091/mbc.E23-06-0255. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
214472MCAM-BirA*-HALentiviral vector for the expression of MCAM-BirA*-HA fusion protein in mammalian cells.
214473BirA*-HA-NESLentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.
214474Lyn11-BirA*-HALentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with an N-terminal Lyn11 plasma membrane localization sequence and C-terminal HA tag.
214475MCAM-GFPLentiviral vector for the expression of MCAM-GFP fusion protein in mammalian cells.
214476RDX-mCherryPlasmid for transient transfection of RDX-mCherry fusion protein in mammalian cells.
214477mCherry-VectorVector control for mCherry fusions. Created from the backbone of Addgene #55043 mCherry-Ezrin-N-14 from Michael Davidson.

Antibodies from Article