A fluorogenic complementation tool kit for interrogating lipid droplet-organelle interaction.
Li X, Gamuyao R, Wu ML, Cho WJ, King SV, Petersen RA, Stabley DR, Lindow C, Climer LK, Shirinifard A, Ferrara F, Throm RE, Robinson CG, Zhou Y, Carisey AF, Tebo AG, Chang CL
J Cell Biol. 2024 Sep 2;223(9):e202311126. doi: 10.1083/jcb.202311126. Epub 2024 Jul 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214409 | mApple-1xHp | Lipid droplet and ER localization of 1xHp of Spastin fused to mApple |
| 214410 | mApple-6xHp | Lipid droplet localization of 6xHp of Spastin fused to mApple |
| 214411 | APEX2-6xHp | Lipid droplet localization of APEX2-6xHp for electron microscopy |
| 214412 | NFAST(low)-mApple-6xHp | Lipid droplet localization of low-affinity NFAST fused to mApple-6xHp |
| 214413 | CFAST-Halotag-ER | ER localization of CFAST fused to Halotag-ER |
| 214414 | Halotag-6xHp | Lipid droplet localization of 6xHp of Spastin fused to Halotag |
| 214415 | NFAST(low)-mApple-mito | Mitochondria localization of low-affinity NFAST fused to mApple-mito |
| 214416 | CFAST-Halotag-6xHp | Lipid droplet localization of CFAST fused to Halotag-6xHp |
| 214417 | PMP34-mApple | Peroxisome localization of PMP34 fused to mApple |
| 214418 | PMP34-mApple-NFAST(low) | Peroxisome localization of low-affinity NFAST fused to PMP34-Apple |
| 214419 | NFAST(low)-Halo-ER | ER localization of low-affinity NFAST fused to Halo-ER |
| 214420 | Lenti-FAB(ER-LD) | Detection of ER-lipid droplet contact site |
| 214421 | Lenti-FAB(mito-LD) | Detection of mito-lipid droplet contact site |
| 214422 | Lenti-FAB(PX-LD) | Detection of peroxisome-lipid droplet contact site |
| 214423 | Lenti-FAB(ER-mito) | Detection of ER-mito contact site |
| 214424 | FAB(ER-LD) | Detection of ER-lipid droplet contact site |
| 214425 | FAB(mito-LD) | Detection of mito-lipid droplet contact site |
| 214426 | FAB(PX-LD) | Detection of peroxisome-lipid droplet contact site |
| 214427 | FAB(ER-mito) | Detection of ER-mito contact site |
| 217376 | Lenti-mApple-6xHp | Lipid droplet localization of 6xHp of Spastin fused to mApple |
| 217377 | mApple-ERM | ER marker |