Combination of AID2 and BromoTag expands the utility of degron-based protein knockdowns.
Hatoyama Y, Islam M, Bond AG, Hayashi K, Ciulli A, Kanemaki MT
EMBO Rep. 2024 Aug 23. doi: 10.1038/s44319-024-00224-4.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214373 | pMK382 | Tet-OsTIR1(F74G) (AAVS1 insertion donor) |
| 214374 | pMK444 (EF1-OsTIR1(F74G) Puro) | EF1-OsTIR1(F74G) (PiggyBac) |
| 214375 | pMK445 (BSD-P2A-BromoTag) | BromoTag for N-terminal tagging |
| 214376 | pMK446 (BSD-P2A-BromoTag-mClover) | BromoTag-mClover for N-terminal tagging |
| 214377 | pMK447 (BSD-P2A-BromoTag-mCherry) | BromoTag-mCherry for N-terminal tagging |
| 214378 | pMK448 (BromoTag-BSD) | BromoTag for C-terminal tagging |
| 214379 | pMK449 (BromoTag-mClover-BSD) | BromoTag-mClover for C-terminal tagging |
| 214380 | pMK450 (BromoTag-mCherry-BSD) | BromoTag-mCherry for N-terminal tagging |
| 214381 | pMK451 (BSD-P2A-dTAG) | dTAG for N-terminal tagging |
| 214382 | pMK452 (BSD-P2A-dTAG-mClover) | dTAG-mClover for N-terminal tagging |
| 214383 | pMK453 (BSD-P2A-dTAG-mCherry) | dTAG-mCherry for N-terminal tagging |
| 214384 | pMK454 (dTAG-BSD) | dTAG for C-terminal tagging |
| 214385 | pMK455 (dTAG-mClover-BSD) | dTAG-mClover for C-terminal tagging |
| 214386 | pMK456 (dTAG-mCherry-BSD) | dTAG-mCherry for C-terminal tagging |
| 214387 | pMK459 (dTAG-BromoTag-mAID-EGFP) | dTAG-BromoTag-mAID-EGFP reporter (PiggyBac) |
| 214388 | pMK462 (Hygro-P2A-mAB) | mAID-BromoTag for N-terminal tagging |
| 214389 | pMK463 (BSD-P2A-mAB) | mAID-BromoTag for N-terminal tagging |
| 214390 | pMK464 (mAB-Neo) | mAID-BromoTag for C-terminal tagging |
| 214391 | pMK465 (mAB-Hygro) | mAID-BromoTag for C-terminal tagging |
| 214392 | pMK466 (mAB-BSD) | mAID-BromoTag for C-terminal tagging |
| 214393 | pMK467 (dTAG-BromoTag-mAID-EGFP) | dTAG-BromoTag-mAID-EGFP reporter (TOL2) |
| 214394 | pMK468 (BSD-P2A-mAID-dTAG) | mAID-dTAG for N-terminal tagging |
| 214395 | pMK469 (mAID-dTAG BSD) | mAID-dTAG for C-terminal tagging |
| 214396 | pMK484 (2mAID-Hygro) | 2mAID for N-terminal tagging |
| 214397 | pMK485 (3mAID-Hygro) | 3mAID for N-terminal tagging |
| 214398 | pMK486 (Hygro-P2A-2mAID) | 2mAID for C-terminal tagging |
| 214399 | pMK487 (Hygro-P2A-3mAID) | 3mAID for C-terminal tagging |