Phage-assisted evolution of compact Cas9 variants targeting a simple NNG PAM.
Qi T, Wang Y, Yang Y, Gao S, Liu J, Huang Q, Tian Y, Tang J, Zheng WV, Wang Y
Nat Chem Biol. 2023 Dec 5. doi: 10.1038/s41589-023-01481-5.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214347 | pBT120a17-sg-del | Expresses pIII |
| 214348 | MP6-SlugABEN-738 | Expresses N-terminus of ABE, and untargeted Bacterial Mutagenesis |
| 214350 | pSK-Sasg | Expresses cloning backbone for SlugCas9 sgRNA |
| 214351 | pSK-Spsg | Expresses cloning backbone for SpCas9 sgRNA |
| 214352 | pCMV-SlugCas9-NNG-P2A-puro | Expresses SlugCas9-NNG and puromycin resistance genes |
| 214353 | pCMV-SlugCas9-P2A-puro | Expresses SlugCas9 and puromycin resistance genes |
| 214354 | pCMV-SlugABE-NNG-P2A-puro | Expresses SlugABE-NNG and puromycin resistance genes |
| 214355 | pCMV-SlugCBE-NNG-P2A-puro | Expresses SlugCBE-NNG and puromycin resistance genes |
| 214356 | pAAV-SlugABE-NNG | Expresses SlugABE-NNG |
| 214357 | pCMV-SpCas9HF1-P2A-puro | Expresses SpCas9HF1 and puromycin resistance genes |
| 214358 | pCMV-SpCas9-P2A-puro | Expresses SpCas9 and puromycin resistance genes |