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Structural insights into cytokine cleavage by inflammatory caspase-4.

Devant P, Dong Y, Mintseris J, Ma W, Gygi SP, Wu H, Kagan JC
Nature. 2023 Dec;624(7991):451-459. doi: 10.1038/s41586-023-06751-9. Epub 2023 Nov 22. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
214305pMSCV-Myc-Caspase-5Expression of gene in mammalian cells by retroviral transduction
214306pET28_Caspase-5-enzymatic-domainProtein overexpression in bacteria
214307pET28_Caspase-4_p20_C258AProtein overexpression in bacteria
214308pET16_Caspase-4_p10Protein overexpression in bacteria
214309pMSCV-Myc-Caspase-4_K356DExpression of gene in mammalian cells by retroviral transduction
214310pMSCV-Myc-Caspase-4_R269DExpression of gene in mammalian cells by retroviral transduction
214311pMSCV-Myc-Caspase-4_W267NExpression of gene in mammalian cells by retroviral transduction
214312pET28_Caspase-4_K356DProtein overexpression in bacteria
214313pET28_Caspase-4_R269DProtein overexpression in bacteria
214314pET28_Caspase-4_W267NProtein overexpression in bacteria
214315pET28_Caspase-11_humanized-mutantProtein overexpression in bacteria
214316pMSCV-Myc-Caspase-11_humanized-mutantExpression of gene in mammalian cells by retroviral transduction
214317pMSCV-human-pro-IL-18Expression of gene in mammalian cells by retroviral transduction
214318pMSCV-human-pro-IL-18_E192K_D193KExpression of gene in mammalian cells by retroviral transduction
214319pMSCV-human-pro-IL-18_V47N_I48NExpression of gene in mammalian cells by retroviral transduction
214320pMSCV-human-pro-IL-18_DDEN29ENGDExpression of gene in mammalian cells by retroviral transduction
214321pMSCV-human-pro-IL-18_LES33AAAExpression of gene in mammalian cells by retroviral transduction
214322pET28_human-pro-IL-18_E192K_D193KProtein overexpression in bacteria
214323pET28_human-pro-IL-18_I48NProtein overexpression in bacteria
214324pET28_human-pro-IL-18_V47N_I48NProtein overexpression in bacteria
214325pET28_human-pro-IL-18_DDEN29ENGDProtein overexpression in bacteria
214326pET28_human-pro-IL-18_LES33AAAProtein overexpression in bacteria
214327pET28_human-pro-IL-18_DeltaN24Protein overexpression in bacteria
214328pET28a_Caspase-4_lemur_catalytic domain Protein overexpression in bacteria
214329pET28a_Caspase-11_rat_catalytic domain Protein overexpression in bacteria
214330pET28a_Caspase-4_rabbit_catalytic domain Protein overexpression in bacteria
214331pET28a_Caspase-4_sheep_catalytic domain Protein overexpression in bacteria
214332pET28a_Pro-IL-18_lemurProtein overexpression in bacteria
214333pET28a_Pro-IL-18_ratProtein overexpression in bacteria
214334pET28a_Pro-IL-18_rabbitProtein overexpression in bacteria
214335pET28a_Pro-IL-18_sheepProtein overexpression in bacteria
214336pET28a_Pro-IL-18_dogProtein overexpression in bacteria
214337pFastBac_human-pro-IL-18Protein overexpression in bacteria
214338pFastBac_human-IL-18Protein overexpression in bacteria
214339pET28a_mouse-Pro-IL-18_humanized-triple-mutantProtein overexpression in bacteria
214340pET28a_Pro-IL-1βProtein overexpression in bacteria
214341pET28a_Pro-IL-1αProtein overexpression in bacteria
214342pET28a_Pro-IL-33Protein overexpression in bacteria
214343pET28a_Pro-IL-36αProtein overexpression in bacteria
214344pET28a_Pro-IL-36βProtein overexpression in bacteria
214345pET28a_Pro-IL-36γProtein overexpression in bacteria
214346pET28a_Pro-IL-37Protein overexpression in bacteria

Antibodies from Article