Standardized Parts for Activation of Small GTPase Signaling in Living Cells.
He Y, Faulkner BM, Roberti MA, Bassford DK, Stains C
Angew Chem Int Ed Engl. 2024 Jul 26:e202403499. doi: 10.1002/anie.202403499.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214278 | pIRES-mCerulean-FRB-Cdc42/N12-Cdc42/13C(Q61L)-FKBP-mVenus-CAAX | Express split-Cdc42 fragments that are fused with CIDs and FPs |
| 214279 | pIRES-mCerulean-FRB-Cdc42/N12-Cdc42/13C(T17N)-FKBP-mVenus-CAAX | Express split-Cdc42 fragments that are fused with CIDs and FPs |
| 214280 | pIRES-mCerulean-FRB-Rac1/N12-Rac1/13C(Q61L)-FKBP-mVenus-CAAX | Express split-Rac1 fragments that are fused with CIDs and FPs |
| 214281 | pIRES-mCerulean-FRB-Rac1/N12-Rac1/13C(T17N)-FKBP-mVenus-CAAX | Express split-Rac1 fragments that are fused with CIDs and FPs |
| 214282 | pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(Q63L)-FKBP-mVenus-CAAX | Express split-RhoA fragments that are fused with CIDs and FPs |
| 214283 | pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(T19N)-FKBP-mVenus-CAAX | Express split-RhoA fragments that are fused with CIDs and FPs |
| 214284 | pIRES-mCerulean-FRB-KRas/N12-KRas/13C(Q61L)-FKBP-mVenus-CAAX | Express split-KRas fragments that are fused with CIDs and FPs |
| 214285 | pIRES-mCerulean-FRB-KRas/N12-KRas/13C(S17N)-FKBP-mVenus-CAAX | Express split-KRas fragments that are fused with CIDs and FPs |
| 214286 | pIRES-mCerulean-GID1-KRas/N12-KRas/13C(Q61L)-GAI1-92-mVenus-CAAX | Express split-KRas fragments that are fused with CIDs and FPs |
| 214287 | pIRES-mCerulean-GID1-KRas/N12-KRas/13C(S17N)-GAI1-92-mVenus-CAAX | Express split-KRas fragments that are fused with CIDs and FPs |
| 214288 | pMAL-MBP-Cdc42-Q61L | Express constitutively active Cdc42 fused with MBP |
| 214289 | pMAL-MBP-Cdc42-T17N | Express dominant negative Cdc42 fused with MBP |
| 214290 | pMAL-MBP-FRB-Cdc42/N12 | Express split-Cdc42 fragment fused with FRB and MBP |
| 214291 | pET21b-Cdc42/13C-FKBP-MBP | Express split-Cdc42 fragment fused with FKBP and MBP |
| 214292 | pMAL-MBP-Rac1-Q61L | Express constitutively active Rac1 fused with MBP |
| 214293 | pMAL-MBP-Rac1-T17N | Express dominant negative Rac1 fused with MBP |
| 214294 | pMAL-MBP-FRB-Rac1/N12 | Express split-Rac1 fragment fused with FRB and MBP |
| 214295 | pET21b-Rac1/13C-FKBP-MBP | Express split-Rac1 fragment fused with FKBP and MBP |
| 214296 | pMAL-MBP-RhoA-Q63L | Express constitutively active RhoA fused with MBP |
| 214297 | pMAL-MBP-RhoA-T19N | Express dominant negative RhoA fused with MBP |
| 214298 | pMAL-MBP-FRB-RhoA/N12 | Express split-RhoA fragment fused with FRB and MBP |
| 214299 | pET21b-RhoA/13C-FKBP-MBP | Express split-RhoA fragment fused with FKBP and MBP |
| 214300 | pMAL-MBP-KRas-Q61L | Express constitutively active KRas fused with MBP |
| 214301 | pMAL-MBP-KRas-S17N | Express dominant negative KRas fused with MBP |
| 214302 | pMAL-MBP-FRB-KRas/N12 | Express split-KRas fragment fused with FRB and MBP |
| 214303 | pET21b-KRas/13C-FKBP-MBP | Express split-KRas fragment fused with FKBP and MBP |
| 214304 | pET21b-C-MBP | Provide a bacterial expression vector that encodes C-terminus MBP tag |