ORP9-PH domain-based fluorescent reporters for visualizing phosphatidylinositol 4-phosphate dynamics in living cells
Ajiki M, Yoshikawa M, Miyazaki T, Kawasaki A, Aoki K, Nakatsu F, Tsukiji S
RSC Chemical Biology
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214266 | pCMV-AcGFP1-ORP9-PH | Mammalian expression of green fluorescent PI4P reporter AcGFP1-ORP9-PH |
| 214267 | pCMV-mTagBFP2-ORP9-PH | Mammalian expression of blue fluorescent PI4P reporter mTagBFP2-ORP9-PH |
| 214268 | pCMV-dTomato-ORP9-PH | Mammalian expression of red fluorescent PI4P reporter dTomato-ORP9-PH |
| 214269 | pCMV-iRFP713-ORP9-PH | Mammalian expression of near-IR fluorescent PI4P reporter iRFP713-ORP9-PH |
| 214270 | pPBbsr-AcGFP1-ORP9-PH | Mammalian expression of green fluorescent PI4P reporter AcGFP1-ORP9-PH |
| 214271 | pCMV-GalT-mCherry | Mammalian expression of GalT-mCherry (Golgi marker) |
| 214272 | pCMV-mTagBFP2-FRB-Rab5 | Mammalian expression of mTagBFP2-FRB-Rab5 (early endosome marker) |
| 214273 | pCMV-mTagBFP2-FRB-Rab7 | Mammalian expression of mTagBFP2-FRB-Rab7 (late endosome marker) |
| 214274 | pCMV-LAMP1-mCherry | Mammalian expression of LAMP1-mCherry (lysosome marker) |
| 214275 | pCMV-mTagBFP2-PLCδ-PH | Mammalian expression of blue fluorescent PI(4,5)P2 reporter mTagBFP2-PLCδ-PH |
| 214276 | pCAGGS-HaloTag-KRas4B(CT) | Mammalian expression of HaloTag fused to the C-terminal tail of KRas4B |
| 214277 | pCMV-mScarlet-I-eDHFR-ORP5(ΔPH) | Mammalian expression of ORP5(ΔPH) fused to mScarlet-I-tagged E. coli DHFR |