The membrane-cytoplasmic linker defines activity of FtsH proteases in Pseudomonas aeruginosa clone C.
Mawla GD, Kamal SM, Cao LY, Purhonen P, Hebert H, Sauer RT, Baker TA, Romling U
J Biol Chem. 2024 Feb;300(2):105622. doi: 10.1016/j.jbc.2023.105622. Epub 2024 Jan 3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214257 | pSG071 | Hybrid PaFtsH1NFtsH2 protease: PaftsH2SD -PaftsH21-462-PaftsH1454-1920 |
| 214258 | pSG075 | Hybrid PaFtsH2NFtsH1 protease: PaftsH2SD -PaftsH11-453-PaftsH2463-1881 in pJN105 |
| 214259 | pSG068 | pJN105 with PaftsH2SD and PaFtsH1 ORF cloned between NheI/XbaI |
| 214260 | pSG069 | pJN105 with PaftsH2SD and PaFtsH2 ORF cloned between NheI/XbaI |
| 214261 | pSG058 | PaFtsH1-6xHis cloned between XbaI/NheI in pET-28a(+) |
| 214262 | pSG059 | PaFtsH2-6xHis cloned in XbaI/NheI site in pET-28a(+) |
| 214263 | pSG086 | PaFtsH2H1-link-32-6xHis: PaftsH2(ftsH2_F124-V154del_ins ftsH1_M120-T151)-6xHis cloned in XbaI/NheI site in pET-28a(+) |