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Saturation mutagenesis of a predicted ancestral Syk-family kinase.

Hobbs HT, Shah NH, Shoemaker SR, Amacher JF, Marqusee S, Kuriyan J
Protein Sci. 2022 Oct;31(10):e4411. doi: 10.1002/pro.4411. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
214235pET23a-H6-TEV-AncSZBacterial expression construct of AncSZ, an kinase domain engineered through ancestorial reconstruction of the kinase domains of both SYK and ZAP-70. For in vitro protein tyrosine phosphorylation

Antibodies from Article