High performance TadA-8e derived cytosine and dual base editors with undetectable off-target effects in plants.
Fan T, Cheng Y, Wu Y, Liu S, Tang X, He Y, Liao S, Zheng X, Zhang T, Qi Y, Zhang Y
Nat Commun. 2024 Jun 14;15(1):5103. doi: 10.1038/s41467-024-49473-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 213469 | pYPQ265E6 | Gateway entry clone (attL1 & attR5) TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing |
| 213470 | pYPQ265E7 | Gateway entry clone (attL1 & attR5) TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing |
| 213471 | pYPQ265E8 | Gateway entry clone (attL1 & attR5) TadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing |
| 213472 | pYPQ265E9 | Gateway entry clone (attL1 & attR5) TadDE-32aa linker-zSpCas9-D10A-2xUGI for C-T and A to G base editing |
| 214250 | pGEL850 | Expression TadCBEa in rice cells |
| 214251 | pGEL851 | Expression TadCBEd in rice cells |
| 214252 | pGEL852 | Expression TadCBEd_V106W in rice cells |
| 214253 | pGEL853 | Expression TadDE in rice cells |
| 214254 | pGEL854 | Expression eTd-CBE in rice cells |
| 214255 | pGEL855 | Expression Td-CBEmax in rice cells |
| 214256 | pGEL856 | Expression TadDE-NG in rice cells |