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Expansion of the split hygromycin toolkit for transgene insertion in Caenorhabditis elegans.

Moerdyk-Schauwecker MJ, Jahahn EK, Munoz ZI, Robinson KJ, Phillips PC
MicroPubl Biol. 2024 Jan 29;2024:10.17912/micropub.biology.001091. doi: 10.17912/micropub.biology.001091. eCollection 2024. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
215674pMS110Split hygromycinR landing pad insertion plasmid for ChrIII
215675pMS18Cas9 + guide plasmid for inserting ChrI split hygromycinR landing pad
215676pMS62Cas9 + guide plasmid for inserting ChrIII split hygromycinR landing pad
215677pMS114Empty repair plasmid for ChrI split hygromycinR landing pad
215678pMS178Empty repair plasmid for ChrII split hygromycinR landing pad
215679pMS158Empty repair plasmid for ChrIII split hygromycinR landing pad
215680pMS59Cas9 + guide plasmid targeting ChrI split hygromycinR landing pad
215681pMS77Cas9 + guide plasmid targeting ChrIII split hygromycinR landing pad
215682pMS160Guide only plasmid targeting chrI split hygromycinR landing pad
215683pMS161Guide only plasmid targeting chrIII split hygromycinR landing pad

Antibodies from Article