Long-term labeling and imaging of synaptically connected neuronal networks in vivo using double-deletion-mutant rabies viruses.
Jin L, Sullivan HA, Zhu M, Lavin TK, Matsuyama M, Fu X, Lea NE, Xu R, Hou Y, Rutigliani L, Pruner M, Babcock KR, Ip JPK, Hu M, Daigle TL, Zeng H, Sur M, Feng G, Wickersham IR
Nat Neurosci. 2024 Jan 11. doi: 10.1038/s41593-023-01545-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 115232 | pLV-TTBG | Lentiviral transfer plasmid for expression of SAD B19 G and mTagBFP2 |
| 115233 | pLV-TTBL | Lentiviral transfer plasmid for expression of SAD B19 L and mTagBFP2 |
| 174378 | pAAV-syn-Flpo | also known as: pAAV-synP-FLPo. AAV virus for expressing FLPo recombinase |
| 178514 | pAAV-syn-F14F15S-jGCaMP7s | AAV vector for Flpo recombinase dependent jGCaMP7s |
| 178515 | pAAV-syn-mCre | AAV vector for mCre recombinase |
| 178516 | pAAV-syn-FLEX-tTA | AAV vector for Cre recombinase dependent tetracycline-controlled transactivator (tTA) expression |
| 178518 | pB-CAG-B19L-IRES-mCherry-WPRE-BGHpA | For making Rabies virus SAD B19 strain L cell line with hyPBase |
| 178519 | TRE-mCardinal-P2A-B19L Flp-in vector | For making TRE-CB mouse line in Tigre locus |
| 183352 | pAAV-syn-F14F15S-splitTVA-EGFP-tTAv2 | Flp-dependent helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene #100799) |