A system of reporters for comparative investigation of EJC-independent and EJC-enhanced nonsense-mediated mRNA decay.
Kolakada D, Campbell AE, Galvis LB, Li Z, Lore M, Jagannathan S
Nucleic Acids Res. 2024 Feb 20:gkae121. doi: 10.1093/nar/gkae121.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214063 | ART | Fluorescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTR |
| 214064 | ARTi | Fluorescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTR |
| 214065 | ARTX_ctrl | Fluorescent reporter system, NMD negative control. No intron in 5'UTR |
| 214066 | ART_RTctrl | Fluorescent reporter system, readthrough control, NMD negative control. No intron in 5'UTR |
| 214067 | AiRT | Fluorescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTR |
| 214068 | AiRTi | Fluorescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTR |
| 214069 | AiRTX_ctrl | Fluorescent reporter system, NMD negative control. Intron present in 5'UTR |
| 214070 | AiRT_RTctrl | Fluorescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTR |
| 214071 | LRT | Luminescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTR |
| 214072 | LRTi | Luminescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTR |
| 214073 | LRTX_ctrl | Luminescent reporter system, NMD negative control. No intron in 5'UTR |
| 214074 | LRT_RTctrl | Luminescent reporter system, readthrough control, NMD negative control. No intron in 5'UTR |
| 214075 | LiRT | Luminescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTR |
| 214076 | LiRTi | Luminescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTR |
| 214077 | LiRTX_ctrl | Luminescent reporter system, NMD negative control. Intron present in 5'UTR |
| 214078 | LiRT_RTctrl | Luminescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTR |