Type III-B CRISPR-Cas cascade of proteolytic cleavages.
Steens JA, Bravo JPK, Salazar CRP, Yildiz C, Amieiro AM, Kostlbacher S, Prinsen SHP, Andres AS, Patinios C, Bardis A, Barendregt A, Scheltema RA, Ettema TJG, van der Oost J, Taylor DW, Staals RHJ
Science. 2024 Feb 2;383(6682):512-519. doi: 10.1126/science.adk0378. Epub 2024 Feb 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214030 | pJS-BCD-Strep-SAVED-CHAT | Bacterial expression plasmid for strep-tagged wild-type SAVED-CHAT from Haliangium ochraceum |
| 214031 | pJS-BCD-Strep-dSAVED-CHAT | Bacterial expression plasmid for strep-tagged catalytically dead SAVED-CHAT mutant from Haliangium ochraceum |
| 214032 | pJS-BCD-Strep-SAVED-CHAT-cbm | Bacterial expression plasmid for strep-tagged SAVED-CHAT cA3-binding pocket mutant from Haliangium ochraceum |
| 214033 | pJS-BCD-Strep-SAVED-CHAT-sm | Bacterial expression plasmid for strep-tagged SAVED-CHAT CHAT-CHAT singlet interface mutant from Haliangium ochraceum |
| 214034 | pJS-BCD-Strep-PCaspase | Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) from Haliangium ochraceum |
| 214035 | pJS-BCD-Strep-dPCaspase | Bacterial expression plasmid for strep-tagged catalytically dead PCaspase (Prokaryotic Caspase) from Haliangium ochraceum |
| 214036 | pJS-BCD-Strep-PCaspase-R153A | Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) R153A mutant from Haliangium ochraceum |
| 214037 | pJS-BCD-Strep-PC-σ | Bacterial expression plasmid for strep-tagged PC-σ from Haliangium ochraceum |
| 214038 | pJS-BCD-Strep-PCi | Bacterial expression plasmid for strep-tagged Pci from Haliangium ochraceum |
| 214039 | pHochTypeIII | Bacterial expression plasmid for Haliangium ochraceum type III CRISPR-Cas complex; contains csb2, a minimal CRISPR array with a pTarget spacer, cmr1-6 |
| 214040 | pSAVED-CHAT_PCaspase_PCi_PC-σ | Bacterial expression plasmid for SAVED-CHAT, PCaspase, PCi, and PC-σ from Haliangium ochraceum |
| 214041 | pSAVED-CHAT | Bacterial expression plasmid for SAVED-CHAT from Haliangium ochraceum |
| 214042 | pPCaspase | Bacterial expression plasmid for PCaspase from Haliangium ochraceum |
| 214043 | pSAVED-CHAT_PCaspase | Bacterial expression plasmid for SAVED-CHAT and PCaspase N-terminal fragment (aa 1-153) from Haliangium ochraceum |
| 214044 | pSAVED-CHAT_PCaspase-N-term | Bacterial expression plasmid for SAVED-CHAT and PCaspase N-terminal fragment (aa 1-153) from Haliangium ochraceum |
| 214045 | pSAVED-CHAT_PCaspase-C-term | Bacterial expression plasmid for SAVED-CHAT and PCaspase C-terminal fragment (aa 154-666) from Haliangium ochraceum |
| 214046 | pSAVED-CHAT_PCaspase-N-C-term | Bacterial expression plasmid for SAVED-CHAT, PCaspase N-terminal fragment (aa 1-153), and C-terminal fragment (154-666) from Haliangium ochraceum |
| 214048 | pSAVED-CHAT-sm_PCaspase | Bacterial expression plasmid for SAVED-CHAT CHAT-CHAT singlet interface mutant and PCaspase from Haliangium ochraceum |
| 214049 | pSAVED-CHAT_PCaspase_PCi | Bacterial expression plasmid for SAVED-CHAT, PCaspase, and PCi from Haliangium ochraceum |
| 214050 | pEcNucC | Bacterial expression plasmid for E. coli MS 115-1 NucC, a cA3-responsive nuclease known to cause abortive infection; used as a positive control in the Haliangium ochraceum type III CRISPR-Cas system |
| 214051 | pEmpty | Empty bacterial expression vector with CloDF13 (CDF) origin of replication; used as a negative control in the Haliangium ochraceum type III CRISPR-Cas system |
| 214052 | pTarget | Expresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer. |
| 214053 | pNon-target | Expresses non-target sequence for Haliangium type III CRISPR-Cas complex; identical to pTarget (Addgene plasmid # 214052) but does not contain a protospacer. |