A robust and inducible precise genome editing via an all-in-one prime editor in human pluripotent stem cells.
Wu Y, Zhong A, Sidharta M, Kim TW, Ramirez B, Persily B, Studer L, Zhou T
bioRxiv [Preprint]. 2024 Jan 19:2024.01.18.576233. doi: 10.1101/2024.01.18.576233.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214019 | AAVS1-iPE2-Hygro | inducible PE2 system for controllable prime editing; this plasmid is used to insert PE2 prime editor in one allele of AAVS1 locus |
| 214020 | AAVS1-iPEmax-Hygro | inducible PEmax system for controllable prime editing; this plasmid is used to insert PEmax prime editor in one allele of AAVS1 locus |
| 214062 | AAVS1-iPE-Plus | inducible PE-Plus system for controllable prime editing; this plasmid is used to insert PE-Plus prime editor (PEmax-P2A-hP53DD-IRES-MLH1dn) in one allele of AAVS1 locus |
| 214079 | pCMV-PE-Plus | Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and IRES-human MLH1dn |
| 214080 | EF1a-hP53DD | Mammalian expression of human P53DD |
| 214082 | EF1a-hP53DD (TET+CTD) | Mammalian expression tetramerization domain (TET) and C-terminal negative regulatory domain (CTD) of human TP53 |
| 214083 | EF1a-hP53DD (TET) | Mammalian expression tetramerization domain (TET) of human TP53 |
| 214084 | pCMV-PEmax-P2A-hP53DD | Mammalian expression of SpCas9 PEmax prime editor with P2A-hP53DD |
| 214085 | lenti-pegRNA-puro backbone | Lentiviral backbone plasmid to insert pegRNAs of interest. The cloning site is BsmbI. |
| 214089 | lenti-tmpknot-epegRNA-puro backbone | Lentiviral backbone plasmid to insert epegRNAs (mpknot) of interest. The cloning site is BsmbI. |
| 214091 | lenti-GBA-N370S-tmpknot-epegRNA | Lentiviral vector expressing epegRNA to induce GBA N370S mutation |
| 214092 | lenti-EGFR-L858R-tmpknot-epegRNA | Lentiviral vector expressing epegRNA to induce EGFR L858R mutation |
| 214093 | lenti-EGFR-T790M-tmpknot-epegRNA | Lentiviral vector expressing epegRNA to induce EGFR T790M mutation |
| 214094 | lenti-KRAS-G12C-tmpknot-epegRNA | Lentiviral vector expressing epegRNA to induce KRAS G12C mutation |
| 214095 | lenti-KRAS-G13A-tmpknot-epegRNA | Lentiviral vector expressing epegRNA to induce KRAS G13A mutation |
| 214096 | lenti-GBA-N370S-nick sgRNA | Lentiviral vector expressing nicking sgRNA to induce GBA N370S mutation using PE3 |
| 214097 | lenti-EGFR-L858R-nick sgRNA | Lentiviral vector expressing nicking sgRNA to induce EGFR L858R mutation using PE3 |
| 214098 | lenti-EGFR-T790M-nick sgRNA | Lentiviral vector expressing nicking sgRNA to induce EGFR T790M mutation using PE3 |
| 214099 | lenti-KRAS-G121C/G13A-nick sgRNA | Lentiviral vector expressing nicking sgRNA to induce KRAS G12C or G13A mutation using PE3 |
| 214100 | lenti-EGFR-L858R-T790M-dual-epegRNA | Lentiviral vector expressing epegRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two epegRNAs using independent U6 promoters. |
| 214101 | lenti-EGFR-L858R-T790M-dual-nick sgRNA | Lentiviral vector expressing nicking sgRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two sgRNAs using independent U6 promoters. |
| 214102 | pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn | Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dn |