A high-throughput synthetic biology approach for studying combinatorial chromatin-based transcriptional regulation

Alcantar MA, English MA, Valeri JA, Collins JJ
Molecular Cell, June 2024 Article

Plasmids from Article

ID	Plasmid	Purpose
213834	pReporter_ZF2_ZF1_mNeonGreen	Facilitates genomic integration of the reporter for screen 1: ZF-1 followed by ZF-2 upstream of the reporter gene.
213835	pReporter_ZF1_mNeonGreen_ZF2	Facilitates genomic integration of the reporter for screen 2: ZF-1 upstream of the reporter gene and ZF-2 downstream.
213836	pReporter_ZF1_ZF2_mNeonGreen	Facilitates genomic integration of the reporter for screen 3: ZF-2 followed by ZF-1 upstream of the reporter gene.
213837	pReporter_ZF2_mNeonGreen_ZF1	Facilitates genomic integration of the reporter for screen 4: ZF-2 upstream of the reporter gene and ZF-1 downstream.
213838	pReporter_mNeonGreen	Facilitates genomic integration of the control reporter without zinc finger binding sites.
213839	pPtet-1_mNeonGreen_Ptet-2_mCherry	Expression of mNeonGreen and mCherry from aTc-inducible Ptet promoters.
213840	pReporter_ZF1_mNeonGreen	Facilitates genomic integration of the reporter with upstream binding sites for ZF-1
213841	pReporter_ZF2_mNeonGreen	Facilitates genomic integration of the reporter with upstream binding sites for ZF-2
213842	pPtet-2-VP16	Used for expressing VP16 fused to ZF-2.
213843	pPtet-1-VP16	Used for expressing VP16 fused to ZF-1.
213844	pStorage_Ptet-1_ZF1	CombiGEM storage plasmid for ZF-1 CR fusions.
213845	pStorage_Ptet-2_ZF2	CombiGEM storage plasmid for ZF-2 CR fusions.
213846	pLEU	LEU integrating plasmid backbone used to create all of the reporter plasmids.
213847	pReporter_TetR	Facilitates genomic integration of tetR expression cassette.