Bidirectional epigenetic editing reveals hierarchies in gene regulation.
Pacalin NM, Steinhart Z, Shi Q, Belk JA, Dorovskyi D, Kraft K, Parker KR, Shy BR, Marson A, Chang HY
Nat Biotechnol. 2024 May 17. doi: 10.1038/s41587-024-02213-3. PubMed Article
Pacalin NM, Steinhart Z, Shi Q, Belk JA, Dorovskyi D, Kraft K, Parker KR, Shy BR, Marson A, Chang HY
Nat Biotechnol. 2024 May 17. doi: 10.1038/s41587-024-02213-3. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 213777 | p101_CRISPRai_PiggyBac | Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector. |
| 213778 | p102_gRNA_Sa_mClover | Cloning vector for Sa gRNA, contains Sa gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and mScarlet for mammalian screening. |
| 213779 | p103_gRNA_Sp_mClover | Cloning vector for Sp gRNA, contains Sp gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and GFP for mammalian screening. |
| 213780 | p104_gRNA_Sp_CD3e | Expresses CRISPRi gRNA for CD3e promoter and GFP. gRNA driven by mU6. |
| 213781 | p105_gRNA_Sa_CD40 | Expresses CRISPRa gRNA for CD40 promoter and mScarlet. gRNA driven by mU6. |