Prime editing using CRISPR-Cas12a and circular RNAs in human cells.
Liang R, He Z, Zhao KT, Zhu H, Hu J, Liu G, Gao Q, Liu M, Zhang R, Qiu JL, Gao C
Nat Biotechnol. 2024 Jan 10. doi: 10.1038/s41587-023-02095-x.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 213743 | pCMV-LbCas12a | For circular RNA-mediated prime editor using LbCas12a in HEK293T cells |
| 213744 | LbCas12a-D156R | For circular RNA-mediated prime editor using LbCas12a-D156R in HEK293T cells |
| 213745 | nLbCas12a | For circular RNA-mediated prime editor using nLbCas12a-D156R-R1138A in HEK293T cells |
| 213746 | rnLbCas12a-PE2 | For circular RNA-mediated prime editor using rnLbCas12a-PE2 in HEK293T cells |
| 213747 | LbCas12a-niCPE | For circular RNA-mediated prime editor using LbCas12a-niCPE in HEK293T cells |
| 213748 | LbCas12a-nuCPE | For circular RNA-mediated prime editor using LbCas12a-nuCPE in HEK293T cells |
| 213749 | MCP-M-MLV RT∆RNase H | For circular RNA-mediated prime editor using MCP-M-MLV RT∆RNase H in HEK293T cells |
| 213750 | U6-5' MS2-CircRNA | For circular RNA-mediated prime editor using U6-5' MS2-CircRNA in HEK293T cells |
| 213751 | U6-3' MS2-CircRNA | For circular RNA-mediated prime editor using U6-3' MS2-CircRNA in HEK293T cells |
| 213752 | U6-5'+3' MS2-CircRNA | For circular RNA-mediated prime editor using U6-5'+3' MS2-CircRNA in HEK293T cells |
| 213753 | LbCas12a-crRNA | For circular RNA-mediated prime editor using LbCas12a-crRNA in HEK293T cells |