Synaptotagmin-7 outperforms synaptotagmin-1 to promote the formation of large, stable fusion pores via robust membrane penetration.
Courtney KC, Mandal T, Mehta N, Wu L, Li Y, Das D, Cui Q, Chapman ER
Nat Commun. 2023 Nov 27;14(1):7761. doi: 10.1038/s41467-023-42497-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 213619 | pET SUMO-syt1 (96-421) | Bacterial expression of SUMO-tagged rat syt1 (96-421) |
| 213620 | pET SUMO-syt1 (96-421) I367C | Bacterial expression of SUMO-tagged rat syt1 (96-421) I367C |
| 213621 | pET SUMO-syt7 (136-403) | Bacterial expression of SUMO-tagged mouse syt7 (136-403) |
| 213622 | pET SUMO-syt7 (136-403) L361C | Bacterial expression of SUMO-tagged mouse syt7 (136-403) L361C |
| 213623 | pET SUMO-syt1 5W | Bacterial expression of SUMO-tagged rat syt1 5W |
| 213624 | pET SUMO-syt1 s7+ | Bacterial expression of SUMO-tagged rat syt1 s7+ |
| 213625 | pET SUMO-syt7 C2AB - 4A | Bacterial expression of SUMO-tagged mouse syt7 C2AB - 4A |
| 213626 | pET SUMO-syt7 C2A-2A 2B | Bacterial expression of SUMO-tagged mouse syt7 C2A-2A 2B |
| 213627 | pET SUMO-syt7 C2AB-2A | Bacterial expression of SUMO-tagged mouse syt7 C2AB-2A |
| 213628 | pET SUMO-FL-syt1 | Bacterial expression of SUMO-tagged rat FL-syt1 |
| 213629 | pET SUMO-FL-syt1 5W | Bacterial expression of SUMO-tagged rat FL-syt1 5W |
| 213630 | pET SUMO-FL-syt7 | Bacterial expression of SUMO-tagged mouse FL-syt7 |
| 213631 | pET SUMO-Doc2β C2AB (G361C) | Bacterial expression of SUMO-tagged rat Doc2β C2AB (G361C) |
| 213633 | pET SUMO-cPLA2 C2 domain (Y96C) | Bacterial expression of SUMO-tagged human cPLA2 C2 domain (Y96C) |