A general method for chemogenetic control of peptide function.
Shen J, Geng L, Li X, Emery C, Kroning K, Shingles G, Lee K, Heyden M, Li P, Wang W
Nat Methods. 2023 Jan;20(1):112-122. doi: 10.1038/s41592-022-01697-8. Epub 2022 Dec 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 213530 | pCTCON2-Aga2p-Flag-CapN-TEVcs-HA | Expresses CapN-caged TEVcs on yeast surface |
| 213532 | plx208-CMV-SspB-EGFP-VP16 | Expresses one of the split transcription factor component in mammalian cells |
| 213533 | plx208-CMV-Gal4DBD-CapN-SsrA-CapC | Expresses one of the split transcription factor component in mammalian cells |
| 213535 | pAAV-Synapsin-SspB-EGFP-VP16-P2A-FLAG-TetR-NLS-CapN-SsrA-CapC | Expresses the split transcription factors in cultured neuron and mouse brain |
| 213536 | pAAV-CMV-SspB-EGFP-VP16-P2A-FLAG-Gal4DBD-CapN-SsrA-CapC-HA | Expresses the split transcription factors in mouse liver |
| 213538 | plx208-CMV-Signal seuqence-enkephalin-CapC-FLAG-μOR-NNES-SmBit | Expresses the CapC-caged opioid peptide in mammalian cells |