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pIGLET: Safe harbor landing sites for reproducible and efficient transgenesis in zebrafish.

Lalonde RL, Wells HH, Kemmler CL, Nieuwenhuize S, Lerma R, Burger A, Mosimann C
Sci Adv. 2024 Jun 7;10(23):eadn6603. doi: 10.1126/sciadv.adn6603. Epub 2024 Jun 5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
213509pRL055 pDESTattB_cryaa:VenusattB-containing pDEST plasmid, for use in Multisite Gateway reaction
213510pRL056 pDESTattB_exorh:EGFPattB-containing pDEST plasmid, for use in Multisite Gateway reaction
213511pCK122 pDESTattB_exorh:mCherryattB-containing pDEST plasmid, for use in Multisite Gateway reaction
213512pCK123 pDESTattB_exorh:mCeruleanattB-containing pDEST plasmid, for use in Multisite Gateway reaction
213513pRL092 pattB_cryaa:Venus_MCSattB-containing vector backbone, for use in restriction enzyme- or PCR-based cloning
213514pRL093 pattB_exorh:EGFP_MCSattB-containing vector backbone, for use in restriction enzyme- or PCR-based cloning

Antibodies from Article