Molecular basis and cellular functions of vinculin-actin directional catch bonding.
Chirasani VR, Khan MAI, Malavade JN, Dokholyan NV, Hoffman BD, Campbell SL
Nat Commun. 2023 Dec 14;14(1):8300. doi: 10.1038/s41467-023-43779-x.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 213411 | pRRL-VcnTS-E1015A-E1021A | Vinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A), in lentiviral expression vector. |
| 213412 | pcDNA3.1-VcnCS-E1015A | Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A). |
| 213413 | pcDNA3.1-VcnCS-E1021A | Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A). |
| 213414 | pcDNA3.1-VcnCS-E1015A-E1021A | Vinculin conformation sensor (VcnCS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A). |
| 213415 | pcDNA3.1-VcnTS-E1015A | Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A). |
| 213416 | pcDNA3.1-VcnTS-E1021A | Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A). |
| 213417 | pcDNA3.1-VcnTS-E1015A-E1021A | Vinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A). |