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Multi-label in vivo STED microscopy by parallelized switching of reversibly switchable fluorescent proteins.

Willig KI, Wegner W, Muller A, Calvet-Fournier V, Steffens H
Cell Rep. 2021 Jun 1;35(9):109192. doi: 10.1016/j.celrep.2021.109192. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
213398pAAV-ZFN-hSyn-DIO-PSD95-FingR-Citrine-CCR5TCtranscriptionally regulated, double floxed, postsynaptic marker of PSD95 fused to Citrine
213399pAAV-hSyn-DIO-myr-rsEGFP2- LDLRdouble floxed, myristoylation site (myr) and LDLR, fused to reversibly switchable EGFP rsEGFP2

Antibodies from Article