Modularly assembled multiplex prime editors for simultaneous editing of agronomically important genes in rice.
Gupta A, Liu B, Raza S, Chen QJ, Yang B
Plant Commun. 2023 Oct 26:100741. doi: 10.1016/j.xplc.2023.100741.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 213049 | pG3H-PE3max-attR1R2 | Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites |
| 213050 | pAG79-attL1-pegRNA-attL2 | Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attL2 |
| 213051 | pCR8-attL1-pegRNA-attR3 | Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR3 |
| 213052 | pCR8-attL3-pegRNA-attL2 | Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL3 and attL2 |
| 213053 | pCR8-attL1-pegRNA-attR5 | Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5 |
| 213054 | pCR8-attL5-pegRNA-attR3 | Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attR3 |
| 213055 | pCR8-attL5-pegRNA-attL4 | Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attL4 |
| 213056 | pCR8-attR4-pegRNA-attR3 | Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attR4 and attR3 |