Oliver Weichenrieder Lab:
Structures, Functions, and Adaptations of the Human LINE-1 ORF2 Protein
Unpublished
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 213024 | pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41 | Expresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusion |
| 213025 | pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1 | Expresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cells |
| 213026 | pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro | Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2 |
| 213028 | pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646) | Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2 |
| 213029 | pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646) | Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2 |
| 213030 | pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro | Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2 |